2010 Fiscal Year Final Research Report
In vivo imaging of osteoclast precursor recruitment to the inflammatory site where extensive bone destruction occurs
| Project/Area Number |
20592185
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Showa University |
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Principal Investigator |
SUZUKI Keiko Showa University, 歯学部, 講師 (50119187)
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| Co-Investigator(Kenkyū-buntansha) |
YAMADA Shoji 昭和大学, 歯学部, 教授 (00111617)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 炎症性骨破壊 / 破骨細胞 / イメージング / 骨吸収治療薬 |
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| Research Abstract |
In this study, we investigated the mobilization of osteoclast precursor cells (OCPs) in living mice, in which the interrelationship among different cell types are maintained, by using in vivo imaging techniques. Within 3 days of luciferase (Luc)-Tg OCPs injection, Luc-positive cells were detected in the inflammation site, secondary lymph nodes and spleen; and furthermore, expression of TNFalpha, IL-1beta, IL-6, MCP-1 and MIP-1alpha mRNAs were significantly increased in LPS- or Pam3-injected mice. These findings indicate that OCPs circulating in the bloodstream migrate into the local inflammation site through MCP-1/MIP-1alpha activation and can take part in the rapid and extensive bone destruction.
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