The functional elucidation of salivary protein histatin toward the development of anti-inflammatory agent

2010 Fiscal Year Final Research Report

• Project/Area Number: 20592210
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Matsumoto Dental University
• Principal Investigator: IMAMURA Yasuhiro Matsumoto Dental University, 歯学部, 講師 (00339136)
• Co-Investigator(Kenkyū-buntansha): FUJIGAKI Yoshihisa 松本歯科大学, 歯学部, 助教 (80367523) ; ARA Toshiaki 松本歯科大学, 歯学部, 助教 (90387423) ; FUJINAMI Yoshiaki 松本歯科大学, 歯学部付属病院, 助手 (80392801) ; WANG PaoーLi 大阪歯科大学, 歯学部, 教授 (20213613)
• Project Period (FY): 2008 – 2010
• Keywords: 唾液蛋白質 / ヒスタチン / 炎症 / 細胞周期

Research Abstract

Salivary protein histatin has antimicrobial activity toward pathogenic bacteria of periodontitis. However, the effect of histatin on living body (host) is scarcely clarified. In this study, histatin showed the enhancement of human gingival fibroblast (which constitute the major cellular population of gingival tissue) proliferation. It became apparent that histatin is an anti-inflammatory factor. These observations suggest that wound healing induced by inflammatory, etc., in the oral cavity is earlier than that in the other tissues and organs. Moreover, it seems more likely that the salivary protein is developed as an anti-inflammatory agent.

Research Products

• [Journal Article] Transcriptional regulation of the salivary histatin gene : Finding of a strong positive regulatory element and its binding protein. (2009)
  • Author(s): Imamura Y., Fujigaki Y., Oomori Y., Ouryouji K., Yanagisawa S., Miyazawa H., Wang P.-L.
  • Journal Title: J.Biochem. Vol.145 Pages: 279-288
  • Peer Reviewed
• [Journal Article] Cooperation of salivary protein histatin 3 with heat shock cognate protein 70 relative to the G1/S transition in human gingival fibroblasts. (2009)
  • Author(s): Imamura Y., Fujigaki Y., Oomori Y., Usui S., Wang P.-L.
  • Journal Title: J.Biol.Chem. Vol.284 Pages: 14316-14325
  • Peer Reviewed
• [Presentation] 熱ショック蛋白質を介したTLR4シグナルにおける唾液ヒスタチンの抑制効果 (2010)
  • Author(s): 今村泰弘
  • Organizer: 第52回歯科基礎医学会学術大会ならびに総会
  • Place of Presentation: 東京都タワーホール船堀
  • Year and Date: 2010-09-22
• [Presentation] 唾液ヒスタチンによるヒト歯肉線維芽細胞の細胞周期制御 (2009)
  • Author(s): 今村泰弘
  • Organizer: 第51回歯科基礎医学会学術大会ならびに総会
  • Place of Presentation: 新潟県新潟市朱鷺メッセ
  • Year and Date: 2009-09-11
• [Presentation] ヒト歯肉線維芽細胞の細胞周期に関与する唾液ヒスタチンの機能 (2008)
  • Author(s): 今村泰弘
  • Organizer: 第50回歯科基礎医学会学術大会ならびに総会
  • Place of Presentation: 東京都TOC有明
  • Year and Date: 2008-09-25
• [Remarks] 読売新聞長野版,2009年6月27日,朝刊,口腔内の傷回復の仕組み解明たんぱく質結合歯肉細胞が増殖,松本歯科大