Defense mechanisms of periapical inflammation by endothelial cells in persistent apical periodontitis

2010 Fiscal Year Final Research Report

• Project/Area Number: 20592240
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Nihon University
• Principal Investigator: TAKEICHI Osamu Nihon University, 歯学部, 講師 (10277460)
• Project Period (FY): 2008 – 2010
• Keywords: 慢性根尖性歯周炎 / 歯根肉芽種 / 歯根嚢胞 / 血管内皮細胞 / 一酸化窒素 / 糖化最終産物受容体 / midkine / ケモカイン

Research Abstract

To determine the mechanisms of persistent apical periodontitis, nitric oxide (NO), receptor for advanced glycation end products (RAGE) and midkine were analyzed using immunohistochemical and molecular biological techniques. NO and midkine were co-expressed by endothelial cells. RAGE was also expressed by AGE- expressing endothelial cells. In addition, co-expression of midkine and chemokines by endothelial cells were determined. The data suggested that endothelial cells could play a central role in extension and defense of periapical inflammation.

Research Products

• [Journal Article] Midkine expression in human periapical granulomas (2011)
  • Author(s): Hatori K, Takeichi O, Ogiso B, Maeno M, Komiyama K
  • Journal Title: Journal of Endodontics 37(in press)
  • Peer Reviewed
• [Presentation] RAGE-AGE interactions are important in periapical granulomas (2010)
  • Author(s): Takeichi O, Hatori K, Ogiso B, Saito I
  • Organizer: 88th General Session and Exhibition of the IADR
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 20100714-20100717
• [Presentation] Midkine is expressed in human periapical granulomas (2010)
  • Author(s): Hatori K, Takeichi O, Ogiso B
  • Organizer: 88th General Session and Exhibition of the IADR
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 20100714-20100717
• [Presentation] 歯根肉芽種のおけるmidkine遺伝子発現 (2010)
  • Author(s): 羽鳥啓介、武市収、他
  • Organizer: 第132回春季日本歯科保存学会
  • Place of Presentation: 崇城大学市民ホール、熊本市、熊本県
  • Year and Date: 20100604-20100605
• [Presentation] 歯根肉芽種におけるmidkine発現とその機能的役割について (2010)
  • Author(s): 羽鳥啓介、武市収、小木曾文内
  • Organizer: 第62回日本大学歯学会総会
  • Place of Presentation: 日本大学歯学部、千代田区、東京都
  • Year and Date: 2010-05-15
• [Presentation] 歯根肉芽種中の血管内皮細胞によるmidkine及びchemokine発現 (2009)
  • Author(s): 羽鳥啓介、武市収、岩田桜子、勝呂尚、小木曾文内
  • Organizer: 第131回秋季日本歯科保存学会
  • Place of Presentation: 仙台国際センター、仙台市、宮城県
  • Year and Date: 20091029-20091030
• [Presentation] Nitric oxide controls VE-cadherin-mediated vascular integrity in periapical granulomas (2008)
  • Author(s): Takeichi O, Hama S, Fujisaki K, Tanabe N, Hayashi M, Ogiso B, Maeno M, Ochiai K
  • Organizer: 86th General Session and Exhibition of the IADR
  • Place of Presentation: Toronto, ON, Canada
  • Year and Date: 20080703-20080705
• [Presentation] Biocompatibility of novel vacuum sintered bodies of titanium medical apatite (2008)
  • Author(s): Hayashi M, Ogata H, Suzuki N, Komori N, Takeichi O, Tamura K, Morisaki Y, Ogiso B, Fujita T
  • Organizer: 86th General Session and Exhibition of the IADR
  • Place of Presentation: Toronto, ON, Canada
  • Year and Date: 20080703-20080705
• [Remarks] ホームページ等