2010 Fiscal Year Final Research Report
Regeneration of dentin by human skeletal muscle stem cells and developments of new therapies using stem cell injections.
| Project/Area Number |
20592244
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Aichi Gakuin University |
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Principal Investigator |
NAKATA Kazuhiko Aichi Gakuin University, 歯学部, 講師 (70261013)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAMURA Hiroshi 愛知学院大学, 歯学部, 教授 (40064878)
OZEKI Nobuaki 愛知学院大学, 歯学部, 講師 (70469005)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 体性幹細胞 / ヒト骨格筋幹細胞(h-SMSCs) / 象牙芽細胞 / 象牙質再生 |
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| Research Abstract |
We have examined the mechanism of human skeletal muscle stem cells differentiation to odontoblasts for the purpose of dentin regeneration. Morphological analysis indicated that retinoic acid (RA) and bone morphogenetic protein-4 (BMP-4) induced differentiation to the odontogenic pathway. Differentiated cells showed DSPP and Enamelysin; both markers of odontoblast differentiation. We also found significant integrin α1β1, αVβ1 and αVβ3 expression with increased adhesion and motility on collagen type-I substrates. These interactions with the extracellular matrix (ECM) are indicative of dental pulp wound healing. Moreover, differentiated cells was injected showed DSP and osteo-dentin in rats.
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