2010 Fiscal Year Final Research Report

Regeneration of dentin by human skeletal muscle stem cells and developments of new therapies using stem cell injections.

Research Project

Project/Area Number	20592244
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Conservative dentistry
Research Institution	Aichi Gakuin University
Principal Investigator	NAKATA Kazuhiko Aichi Gakuin University, 歯学部, 講師 (70261013)
Co-Investigator(Kenkyū-buntansha)	NAKAMURA Hiroshi 愛知学院大学, 歯学部, 教授 (40064878) OZEKI Nobuaki 愛知学院大学, 歯学部, 講師 (70469005)
Project Period (FY)	2008 – 2010
Keywords	体性幹細胞 / ヒト骨格筋幹細胞(h-SMSCs) / 象牙芽細胞 / 象牙質再生
Research Abstract	We have examined the mechanism of human skeletal muscle stem cells differentiation to odontoblasts for the purpose of dentin regeneration. Morphological analysis indicated that retinoic acid (RA) and bone morphogenetic protein-4 (BMP-4) induced differentiation to the odontogenic pathway. Differentiated cells showed DSPP and Enamelysin; both markers of odontoblast differentiation. We also found significant integrin α1β1, αVβ1 and αVβ3 expression with increased adhesion and motility on collagen type-I substrates. These interactions with the extracellular matrix (ECM) are indicative of dental pulp wound healing. Moreover, differentiated cells was injected showed DSP and osteo-dentin in rats.

(3 results)