2010 Fiscal Year Final Research Report
Characterization of putative amelogenic cells and mesenchymal cells isolated from human dental follicle and dental papilla
| Project/Area Number |
20592267
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Prosthetic dentistry
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| Research Institution | Okayama University |
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Principal Investigator |
KANYAMA Manabu Okayama University, 岡山大学病院, 講師 (90294420)
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| Co-Investigator(Kenkyū-buntansha) |
KUBOKI Takuo 岡山大学, 大学院・医歯薬学総合研究科, 教授 (00225195)
SONOYAMA Wataru 岡山大学, 岡山大学病院, 助教 (40325121)
KATAOKA Ken 岡山大学, 大学院・医歯薬学総合研究科, 助教 (10293317)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 上皮間葉相互作用 / 象牙質再生 |
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| Research Abstract |
In this study, we isolated human dental follicle epithelial (DFE) cells that were different from gingival epithelial (GE) cells in terms of their gene expression profiles and proliferative capacity. Furthermore, DFE cells were revealed to be putative amelogenic cells based on their gene expression of an ameloblast marker, amelogenin. We found that a direct contact between DFE and dental papilla mesenchymal (DPM) cells increased an amelogenin expression in DFE cells, suggesting transmembrane signaling from the DPM cells played some pivotal roles for DFE cells to enter into the ameloblast lineage. More importantly, this increase was remarkable when DFE cells were cultured with DPM cells in comparison to DFM cells. We confirmed that DPM cells expressed dentin sialophosphoprotein (DSPP), one of odontoblast markers, but DFM cells did not. Therefore, this DPM cells-driven DFE cells differentiation might be a reproduction of epithelial - mesenchymal interaction, found in tooth generation. However, the underlying mechanisms of this induced differentiation of DFE cells were not cleared. Much more signaling works are required to elucidate epithelial - mesenchymal interaction in human tooth generation. Novel human amelogenic cells, DFE cells, might be useful tool for such kind of signaling works.
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