A study of the dental root formation by extracellular environment through Perlecan?SHH interaction.

2010 Fiscal Year Final Research Report

• Project/Area Number: 20592333
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Okayama University
• Principal Investigator: MATSUMURA Tatsushi Okayama University, 岡山大学病院, 助教 (70432648)
• Co-Investigator(Kenkyū-buntansha): HIRATA Azumi 大阪大学, 大学院・工学研究科, 特任研究員 (40263587) ; MISHIMA Katsuaki 山口大学, 大学院・医学系研究科, 准教授 (60304317) ; YAMADA Tomohiro 高知大学, 教育研究部, 講師 (60335619) ; MORITANI Norifumi 岡山大学, 大学院・医歯学総合研究科, 助教 (60467751) ; TABATA Jyun 東京医科歯科大学, 大学院・医歯学総合研究科, 准教授 (20243248)
• Project Period (FY): 2008 – 2010
• Keywords: 歯根形成 / パールカン / SHH / PTCH / SMOH / ヘパラナーゼ

Research Abstract

While perlecan disappers from basal membrane of Hertwig's Epithelial Root Sheath (HERS) along with a progression of dental root formation, heparanase which is one of the digestion enzymes of perlecan migrates to epithelium cells of HERS. During the digestion process of perlecan by heparanase, Sonic Hedgehog (SHH) is cleavaged as a secreted protein. Therefore, we hypothesized that SSH might play a crucial role to establish dental roots.Despite the fact that many approaches have been employed to establish the dental root formation in vitro, it was struggling to reveal this interaction yet. The longer incubation time and the usage of a different type of culture circumstance did not help to improve our goals. More explorations will be essential to achieve the dental root formation by extracellular environment in order to investigate the interaction between perlecan and SHH.

Research Products

• [Journal Article] Mammalian SWI/SNF-A subunit BAF250/ARID1 is an E3 ubiquitin ligase that targets histone H2B2. (2010)
  • Author(s): XS Li, P Trojer, T Matsumura, JE.Treisman, N.Tanese
  • Journal Title: Molecular and Cellular Biology 30(7) Pages: 1673-1688
  • Peer Reviewed
• [Journal Article] Localization of heparanase during palatal bone formation in palatogenesis in mice. (2009)
  • Author(s): A Hirata, T Tsuji, T Ueno, T Yamada, H Imura, T Kagawa, T Matsumura, N Moritani, K Mishima, T Sugahara, N Nakamura
  • Journal Title: Bone 44(2) Pages: S294-295
  • Peer Reviewed
• [Journal Article] Functional requirement of CCN2 for intramembranous bone formation in embryonic mice. (2008)
  • Author(s): Kawaki H, Kubota S, Suzuki A, Yamada T, Matsumura T, Mandai T, Yao M, Maeda T, Lyons KM, Takigawa M.
  • Journal Title: Biochem Biophys Res Commun. 366(2) Pages: 450-456
• [Journal Article] Cooperative regulation of chondrocyte differentiation by CCN2 and CCN3 revealed by a comprehensive analysis of the CCN family proteins in cartilage. (2008)
  • Author(s): H Kawaki, S Kubota, A Suzuki, N Lazar, T Yamada, T Matsumura, T Ohgawara, T Maeda, BP Karen, M Lyons, M Takigawa
  • Journal Title: Journal of Bone and Mineral Research 23(11) Pages: 1751-1764
  • Peer Reviewed
• [Journal Article] Localization of Runx2, osterix, and osteopontin in tooth root formation in rat molars. (2008)
  • Author(s): A Hirata, T Sugahara, H Nakamura
  • Journal Title: J Histochemistry and Cytochemistry 57(4) Pages: 397-403
  • Peer Reviewed
• [Presentation] Localization of heparanase during palatal bone formation in palatogenesis in mice. (2009)
  • Author(s): 平田あずみ, T Tsuji, 植野高章, 山田朋弘, 井村英人, 香川智正, 松村達志, 森谷徳文, 三島克章, 菅原利夫, N Nakamura
  • Organizer: 36^<th> European Symposium on Calcified Tissues
  • Place of Presentation: Vienna
  • Year and Date: 20090523-20090527
• [Presentation] 口蓋形成過程におけるヘパラン硫酸とヘパラナーゼの役割 (2009)
  • Author(s): 平田あずみ、山田朋弘、植野高章、井村英人、香川智正、山近英樹、松村達志、森谷徳文、三島克章、菅原利夫
  • Organizer: 第54回日本口腔外科学会総会
  • Place of Presentation: 札幌
  • Year and Date: 2009-10-10
• [Presentation] Heparanase contribute to palate fusion by degrading perlecan. (2008)
  • Author(s): 平田あずみ, 井村英人, 山田朋弘, 植野高章, E Yamachika, 森谷徳文, 松村達志, 香川智正, 三島克章, 菅原利夫
  • Organizer: XIX Congress of the European Association for Cranio-Maxillo-Facial Surgery.
  • Place of Presentation: Bologna
  • Year and Date: 20080909-20080912
• [Presentation] Localization of Runx2, osterix and osteopontin in cementogenesis in rat molars. (2008)
  • Author(s): 平田あずみ, 植野高章, 香川智正, 松村達志, 山田朋弘, 三島克章, 菅原利夫, H Nakamura
  • Organizer: 35^<th> European Symposium on Calcified Tissues
  • Place of Presentation: Barcelona
  • Year and Date: 20080524-20080528
• [Presentation] ヘパラナーゼは基底膜パールカンよりbFGFを遊離することで口蓋突起癒合に関与する (2008)
  • Author(s): 平田あずみ、井村英人、山田朋弘、植野高章、山近英樹、森谷徳文、松村達志、香川智正、三島克章、菅原利夫
  • Organizer: 第53回日本口腔外科学会総会
  • Place of Presentation: 徳島、ゴールドリボン賞受賞
  • Year and Date: 2008-10-20