2010 Fiscal Year Final Research Report
口腔癌細胞における新規低酸素誘導遺伝子EFEMP2機能解析
| Project/Area Number |
20592335
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
TANIMOTO Keiji Hiroshima University, 原爆放射線医科学研究所, 助教 (90335688)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 低酸素 / 遺伝子 / 発現制御 / 癌 |
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| Research Abstract |
Tumor hypoxia alters expression of various genes via hypoxia-inducible transcription factors, resulting causes numerous changes linked to the biologically aggressive phenotype. We identified EFEMP2 as a novel hypoxia-inducible gene by using oligonucleotide array, and demonstrated that the gene could decrease p53 activity in cancer cells. The expression levels of EFEMP2 were significantly higher in both colon and esophageal cancers compared to the adjacent normal tissues. Promoter reporters showed significant activities in several cancer cells and the activities were increased under hypoxic conditions (1% pO_2) or by cotransfection of HIF-1α. Forced expression of EFEMP2 decreased p53 protein levels and its activity in cancer cells, and also attenuated Notch signaling. Taken together, we demonstrated that EFEMP2 was transcriptionally induced by HIF-1 and its protein regulated cancer cell proliferation via p53 regulations.
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[Journal Article] Basic helix-loop-helix transcription factor DEC1 negatively regulates CyclinD1.2011
Author(s)
Ujjal K.Bhawal, Fuyuki Sato, Yuki Arakawa, Katsumi Fujimoto, Takeshi Kawamoto, Keiji Tanimoto, Yumi Ito, Tomonori Sasahira, Takashi Sakurai, Masaru Kobayashi, Isamu Kashima, Hiroshi Kijima, Hiroki Kuniyasu, Yoshimitsu Abiko, Yukio Kato, Sadao Sato.
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Journal Title
Journal of Pathology in press.
Peer Reviewed
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[Journal Article] Activation of the hypoxia-inducible factor-1 in overloaded temporomandibular joint, and induction of osteoclastogenesis.2010
Author(s)
Maya Shirakura, Keiji Tanimoto, Hideaki Eguchi, Mutsumi Miyauchi, Hideaki Nakamura, Keiko Hiyama, Kotaro Tanimoto, Eiji Tanaka, Takashi Takata, Kazuo Tanne.
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Journal Title
Biochemical and Biophysical Research Communications 393
Pages: 800-805
Peer Reviewed
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[Journal Article] Human mismatch repair gene, MLH1, is transcriptionally repressed by the hypoxia-inducible transcription factors, DEC1 and DEC2.2008
Author(s)
Hideaki Nakamura, Keiji Tanimoto, Mayu Yunokawa, Takeshi Kawamoto, Yukio Kato, Koji Yoshiga, Eiso Hiyama, Lorenz Poellinger, Keiko Hiyama, Masahiko Nishiyama.
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Journal Title
Oncogene 27
Pages: 4200-4209
Peer Reviewed
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[Journal Article] Selection of a novel drug-response predictor in esophageal cancer : A novel screening method using microarray and identification of IFITM1 as a potent marker gene of CDDP response.2008
Author(s)
Shoichi Fumoto, Tatsushi Shimokuni, Keiji Tanimoto, Keiko Hiyama, Keiko Otani, Megu Ohtaki, Jun Hihara, Kazuhiro Yoshida, Eiso Hiyama, Tsuyoshi Noguchi, Masahiko Nishiyama.
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Journal Title
International Journal of Oncology 32
Pages: 413-423
Peer Reviewed
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[Presentation] Molecular mechanisms of degradation of the pVHL, tumor suppressor gene product, by a novel oncogenic protein, UBE2S.2009
Author(s)
Keiji Tanimoto, Hideaki Nakamura, Shoichi Fumoto, Eiso Hiyama, Hidetaka Eguchi, Masahiko Nishiyama, Keiko Hiyama.
Organizer
100^<th> Annual Meeting of Am. Assoc. Cancer Res, Denver
Place of Presentation
Colorado
Year and Date
2009-04-21
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[Presentation] Hypoxic signaling in cancers.2009
Author(s)
Keiji Tanimoto, Keiko Hiyama, K.
Organizer
Plenary Symposium, The 7^<th> Scientific Respiratory Medicine Meeting PIPKRA2009
Place of Presentation
Jakarta, Indonesia
Year and Date
2009-02-15
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[Presentation] Diagnostic factors in cancers.2009
Author(s)
Keiji Tanimoto, Keiko Hiyama, K.
Organizer
Satellite Symposium, The 7^<th> Scientific Respiratory Medicine Meeting PIPKRA2009
Place of Presentation
Jakarta, Indonesia
Year and Date
2009-02-14
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[Remarks] ホームページ等
