Novel bone tissue engineering method using PTH signaling, its application to secondary alveolar bone grafting in patients with CLP and orthodontic evaluation

2010 Fiscal Year Final Research Report

• Project/Area Number: 20592387
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: The University of Tokyo
• Principal Investigator: SUSAMI Takafumi The University of Tokyo, 医学部附属病院, 准教授 (80179184)
• Co-Investigator(Kenkyū-buntansha): OHKUBO Kazumi 東京大学, 医学部附属病院, 助教 (10396715) ; OGASAWARA Toru 東京大学, 医学部附属病院, 講師 (20359623) ; OGATA Naoshi 東京大学, 医学部附属病院, 講師 (10361495)
• Project Period (FY): 2008 – 2010
• Keywords: 顎裂部骨移植 / 副甲状腺ホルモン / 歯科矯正学

Research Abstract

This study was conducted to (1) make tissue-engineered bone by using parathyroid hormone (PTH), (2) elucidate the mechanism of PTH function in bone formation and (3) evaluate the quality as a material for bone grafting of the tissue-engineered bone constructed by this novel method in terms of orthodontics. As a result, PTH was found to be able to promote bone formation and bone differentiation both in vivo and in vitro. It was suggested that use of PTH or suppression of Gαq signaling may be effective to produce tissue-engineered bone which could be applied to secondary alveolar bone grafting.

Research Products

• [Journal Article] The G&alpha ; q signal in osteoblasts is inhibitory to the osteoanabolic action of Parathyroid Hormone. (2011)
  • Author(s): Ogata N, Shinoda Y, Wettschureck N, Offermans S, Takeda S, Nakamura K, Segre GV, Chung UI, Kawaguchi H.
  • Journal Title: J Biol Chem. 286(15) Pages: 13733-40
  • Peer Reviewed
• [Journal Article] Mechanisms underlying catabolic and anabolic functions of parathyroid hormone on bone by combination of culture systems of mouse cells. (2010)
  • Author(s): Shinoda Y, Kawaguchi H, Higashikawa A, Hirata M, Miura T, Saito T, Nakamura K, Chung UI, Ogata N.
  • Journal Title: J Cell Biochem. 109(4) Pages: 755-63
  • Peer Reviewed
• [Journal Article] Cytokines in bone diseases. Genetic defects of PTH/PTHrP receptor in chondrodysplasia. (2010)
  • Author(s): Ogata N.
  • Journal Title: Clin Calcium.(Review. Japanese.) 20(10) Pages: 1481-8
• [Journal Article] Transforming growth factor-β1 in combination with fibroblast growth factor-2 and insulin-like growth factor-I for chondrocyte proliferation culture and cartilage regenerative medicine. (2009)
  • Author(s): Ogasawara T, Ohba S, Fujihara Y, Takahashi T, Liu G, Chikazu D, Suenaga H, Chung UI, Yoda T, Mori Y, Susami T, Takato T, Hoshi K.
  • Journal Title: Asian J Oral Maxillofac Surg 21 Pages: 18-26
  • Peer Reviewed
• [Journal Article] Screening of chondrogenic factors with a real-time fluorescence-monitoring cell line ATDC5-C2ER : identification of sorting nexin 19 as a novel factor. (2009)
  • Author(s): Kan A, Ikeda T, Saito T, Yano F, Fukai A, Hojo H, Ogasawara T, Ogata N, Nakamura K, Chung UI, Kawaguchi H.
  • Journal Title: Arthritis Rheum. 60(11) Pages: 3314-23
  • Peer Reviewed
• [Journal Article] C/EBPbeta Promotes transition from proliferation to hypertrophic differentiation of chondrocytes through transactivation of p57. (2009)
  • Author(s): Hirata M, Kugimiya F, Fukai A, Ohba S, Kawamura N, Ogasawara T, Kawasaki Y, Saito T, Yano F, Ikeda T, Nakamura K, Chung UI, Kawaguchi H.
  • Journal Title: PLoS One. 4(2) Pages: e4543
  • Peer Reviewed
• [Journal Article] Aptitude of auricular and nasoseptal chondrocytes cultured under a monolayer or three-dimensional condition for cartilage tissue engineering. (2009)
  • Author(s): Asawa Y, Ogasawara T, Takahashi T, Yamaoka H, Nishizawa S, Matsudaira K, Mori Y, Takato T, Hoshi K.
  • Journal Title: Tissue Eng Part A. 15(5) Pages: 1109-18
  • Peer Reviewed
• [Journal Article] Kruppel-like factor 5 causes cartilage degradation through transactivation of matrix metalloproteinase 9. (2008)
  • Author(s): Shinoda Y, Ogata N, Higashikawa A, Manabe I, Shindo T, Yamada T, Kugimiya F, Ikeda T, Kawamura N, Kawasaki Y, Tsushima K, Takeda N, Nagai R, Hoshi K, Nakamura K, Chung UI, Kawaguchi H.
  • Journal Title: J Biol Chem. 283 Pages: 24682-689
  • Peer Reviewed
• [Presentation] PTHによる骨形成促進作用の分子メカニズム (2010)
  • Author(s): 緒方直史, 鄭雄一, 中村耕三, 川口浩
  • Organizer: 第28回日本骨代謝学会学術集会
  • Place of Presentation: 東京都新宿区
  • Year and Date: 20100721-20100723
• [Presentation] Regulator of G protein signaling (RGS)-2 enhances bone anabolic action of PTH through inhibition of Gαq/PKC pathway in osteoblasts. (2009)
  • Author(s): Ogata N, Yano F, Chikazu D, Chung UI, Nakamura K, Kawaguchi H.
  • Organizer: 31th annual meeting of the American Society for Bone and Mineral Research
  • Place of Presentation: Denver, Colorado, USA
  • Year and Date: 20090911-20090915
• [Presentation] Regulator of G protein signaling (RGS)-2はGαq/PKCシグナルを抑制して副甲状腺ホルモン(PTH)の骨同化作用を増強する (2009)
  • Author(s): 緒方直史、矢野文子、鄭雄一、中村耕三、川口浩
  • Organizer: 第27回日本骨代謝学会
  • Place of Presentation: 大阪府大阪市
  • Year and Date: 20090723-20090725
• [Presentation] 細胞周期制御を利用した骨粗鬆症治療法開発のための基礎的検討 (2009)
  • Author(s): 小笠原徹, 高戸毅
  • Organizer: 第9回日本抗加齢医学会総会
  • Place of Presentation: 東京都港区
  • Year and Date: 20090528-20090529
• [Presentation] 片側顎裂に対する二次的骨移植-術後早期の評価と成長終了後の評価- (2009)
  • Author(s): 大久保和美, 須佐美隆史, 松崎雅子, 長濱浩平, 飯野光善, 森良之, 近津大地, 西條英人, 大木明子, 引地尚子, 高戸毅
  • Organizer: 第33回日本口蓋裂学会総会・学術集会
  • Place of Presentation: 東京都千代田区
  • Year and Date: 20090528-20090529
• [Presentation] GαqシグナルはPKCδを介してPTHの骨同化作用を抑制する (2008)
  • Author(s): 緒方直史, 矢野文子, 鄭雄一, 中村耕三, 川口浩
  • Organizer: 第26回日本骨代謝学会学術集会
  • Place of Presentation: 大阪府大阪市
  • Year and Date: 20081029-20081031
• [Presentation] 間葉系細胞の分化多能性維持機構におけるNanogの役割 (2008)
  • Author(s): 小笠原徹, 川口浩, 高戸毅, 星和人
  • Organizer: 第26回日本骨代謝学会学術集会
  • Place of Presentation: 大阪府大阪市
  • Year and Date: 20081029-20081031
• [Presentation] β-カテニンは副甲状腺ホルモン(PTH)受容体の細胞内ドメインに直接結合してそのシグナルを制御する (2008)
  • Author(s): 緒方直史, 矢野文子, 篠田裕介, 鄭雄一, 中村耕三, 川口浩
  • Organizer: 第23回日本整形外科学会基礎学術集会
  • Place of Presentation: 京都府京都市
  • Year and Date: 2008-10-23
• [Book] 医師・歯科医師のための口腔診療必携-困ったときのマニュアル・ヒント集202 (2010)
  • Author(s): 須佐美隆史, ほか
  • Total Pages: 263
  • Publisher: 金原出版
• [Remarks] ホームページ
  • URL: http://plaza.umin.ac.jp/~oralsurg/