2010 Fiscal Year Final Research Report
Basic study on osteoclastogenesis of human periodontal ligament derived cells
| Project/Area Number |
20592417
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Kanagawa Dental College |
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Principal Investigator |
MATSUZAWA Mitsuhiro Kanagawa Dental College, 歯学部, 講師 (60288082)
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| Co-Investigator(Kenkyū-buntansha) |
KIMOTO Shigenari 神奈川歯科大学, 歯学部, 教授 (90205013)
YOKOYAMA Mina 神奈川歯科大学, 歯学部, 助教 (10386849)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 歯根膜細胞 / 骨芽細胞 / 破骨細胞 / 細胞分化 / ECM / アメロジェニン / TRAP |
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| Research Abstract |
The present study examined whether amelogenin activates the cell differentiation in mouse osteoblasts and human periodontal ligament (PDL) cells and used the T7 phage-display osteoblast library to identify a target protein with which amelogenin can interact. The marker genes of osteoblast-phenotype were enhanced by amelogenin in the osteoblasts. However, these genes were suppressed in the PDL cells. The expression of RANKL, a critical regulator of osteoclastogenesis was increased in the osteoblasts and PDL cells. The DNA sequence that demonstrated one of the highest affinities for amelogenin was tissue inhibitor of metalloproteinase-2 (TIMP-2). These findings suggest that amelogenin can induce cell differentiation of osteoblasts and may facilitate the mineralization procedure during matrix formation by increasing the MMP activities.
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