Cell-patterning technology for epithelial rest of Malassez network and its application for periodontal regeneration

2010 Fiscal Year Final Research Report

• Project/Area Number: 20592423
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Periodontal dentistry
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: KOMAKI Motohiro Tokyo Medical and Dental University, 大学院・医歯学総合研究科, 准教授 (30401368)
• Co-Investigator(Renkei-kenkyūsha): MORITA Ikuo 東京医科歯科大学, 大学院・医歯学総合研究科, 教授 (60100129) ; ABE Mayumi 東京医科歯科大学, 大学院・医歯学総合研究科, 教授 (80271980) ; IZUMI Yuichi 東京医科歯科大学, 大学院・医歯学総合研究科, 教授 (60159803)
• Research Collaborator: IWASAKI Kengo 東京医科歯科大学, 大学院・医歯学総合研究科, 助教 (40401351)
• Project Period (FY): 2008 – 2010
• Keywords: 歯周組織再生 / 細胞パターニング / セメント芽細胞

Research Abstract

A periodontal disease is an inflammatory disease due to bacterial infection and is a main cause of tooth loss. Therefore, development of the safe and effective regenerative therapy is an extremely important issue. However, conventional treatment such as the GTR method or the enamel protein does not lead to complete periodontal regeneration. We performed basic evaluation for the purpose of establishment of the periodontal regeneration with the new cementum formation to the root surface and confirmed the effectiveness of the bone regeneration with the cell equipped-carrier and clarified the function of the molecules that participated in cementoblast differentiation.

Research Products

• [Journal Article] Cementum protein 1 (CEMP1) induces a cementoblastic phenotype and reduces osteoblastic differentiation in periodontal ligament cells. (2011)
  • Author(s): Komaki M, Iwasaki K, Arzate H, Sampath Narayanan A, Izumi Y, Morita I
  • Journal Title: J Cell Physiol. in press Pages: PMID:21465469
• [Journal Article] Cell-printing and transfer technology applications for bone defects in mice. (2011)
  • Author(s): Tsugawa J, Komaki M, Yoshida T, Nakahama KI, Amagasa T, Morita I
  • Journal Title: J Tissue Eng Regen Med. in press Pages: PMID:21218471
• [Journal Article] Comparison of characteristics of periodontal ligament cells obtained from outgrowth and enzyme-digested culture methods. (2011)
  • Author(s): Tanaka K, Iwasaki K, Feghali KE, Komaki M, Ishikawa I, Izumi Y
  • Journal Title: Arch Oral Biol. 56(4) Pages: 380-8, PMID:21144495
• [Journal Article] Therapeutic angiogenesis by implantation of a capillary structure constituted of human adipose tissue microvascular endothelial cells. (2010)
  • Author(s): Yoshida T, Komaki M, Hattori H, Negishi J, Kishida A, Morita I, Abe M
  • Journal Title: Arterioscler Thromb Vasc Biol. 30(7) Pages: 1300-1306, PMID:20431071
• [Journal Article] Implantation of capillary structure engineered by optical lithography improves hind limb ischemia in mice. (2010)
  • Author(s): Akahori T, Kobayashi A, Komaki M, Hattori H, Nakahama K, Ichinose S, Abe M, Takeda S, Morita I
  • Journal Title: Tissue Eng Part A. 16(3) Pages: 953-9, PMID:19947885
• [Journal Article] A comparison of the tube forming potentials of early and late endothelial progenitor cells. (2008)
  • Author(s): Mukai N, Akahori T, Komaki M, Li Q, Kanayasu-Toyoda T, Ishii-Watabe A, Kobayashi A, Yamaguchi T, Abe M, Amagasa T, Morita I
  • Journal Title: Exp Cell Res. 314(3)(Epub 2007 Nov 29) Pages: 430-40, PMID:18083163
• [Presentation] 歯根膜幹細胞のpericyteとしての機能解析 (2010)
  • Author(s): 岩崎剣吾, 木村康之, 小牧基浩
  • Organizer: 第53回秋期日本歯周病学会
  • Place of Presentation: サンポートホール高松、香川県
  • Year and Date: 20100918-20100919
• [Presentation] 歯根膜細胞分化におけるcementum protein(CEMP)1発現と機能解析 (2010)
  • Author(s): 小牧基浩、岩崎剣吾, 森田育男
  • Organizer: 第31回日本炎症再生医学会
  • Place of Presentation: 京王プラザホテル、東京
  • Year and Date: 20100805-20100806
• [Presentation] 歯根膜細胞の骨芽細胞分化におけるCP-23発現の検討 (2009)
  • Author(s): 小牧基浩、森田育男、和泉雄一、安部まゆみ
  • Organizer: 第51回春期日本歯周病学会
  • Place of Presentation: 岡山コンベンションセンター、岡山
  • Year and Date: 20090515-20090516
• [Presentation] 歯根膜細胞の骨芽細胞分化におけるCP-23発現の検討 (2008)
  • Author(s): 小牧基浩、津川順一、中浜健一、森田育男
  • Organizer: 第26回日本骨代謝学会
  • Place of Presentation: 大阪国際会議場、大阪
  • Year and Date: 20081029-20081031
• [Presentation] 印刷技術を用いて羊膜に転写した骨芽細胞による骨再生療法 (2008)
  • Author(s): 津川順一、小牧基浩、天笠光雄、森田育男
  • Organizer: 第26回日本骨代謝学会
  • Place of Presentation: 大阪国際会議場、大阪
  • Year and Date: 20081029-20081031
• [Presentation] Cementum protein-23 expression in osteoblastic differentiation of periodontal ligament cells (2008)
  • Author(s): Motohiro Komaki, Kengo Iwasaki, A. Sampath Narayanan, Higinio Arzate, Yuichi Izumi, Mayumi Abe
  • Organizer: 94th Annual Meeting of the American Academy of Periodontology's September 7-9^<th>
  • Place of Presentation: The Washington State Convention & Trade Center, Seattle, WA, USA
  • Year and Date: 20080000