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2009 Fiscal Year Final Research Report

Proteomics of Porphyromonas gingivalis within a model oral microbial community

Research Project

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Project/Area Number 20592453
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Social dentistry
Research InstitutionOsaka University

Principal Investigator

KUBONIWA Masae  Osaka University, 大学院・歯学研究科, 助教 (00303983)

Co-Investigator(Kenkyū-buntansha) MAEDA Kazuhiko  大阪大学, 大学院・歯学研究科, 助教 (00346165)
SEKINE Shinichi  大阪大学, 大学院・歯学研究科, 助教 (70506344)
Project Period (FY) 2008 – 2009
Keywordsバイオフイルム / プロテオミクス / 歯周病原性細菌 / 代謝経路 / ビタミンB
Research Abstract

P.gingivalis can assemble into a heterotypic community with a middle colonizer F.nucleatum and an early colonizer S.gordonii, and that a community lifestyle provides physiologic support for P.gingivalis. 1156 P.gingivalis proteins, including 403 down-regulated and 89 up-regulated proteins, were detected qualitatively during comparison of the three species model community with P.gingivalis incubated alone under the same conditions. Significant decreases were seen in proteins involved in thiamine, cobalamin, and pyrimidine synthesis. These results indicate that a community lifestyle provides physiologic support for P.gingivalis.

  • Research Products

    (22 results)

All 2010 2009 2008

All Journal Article (11 results) (of which Peer Reviewed: 11 results) Presentation (10 results) Book (1 results)

  • [Journal Article] Subgingival biofilm formation2010

    • Author(s)
      Kuboniwa, M., R.J. Lamont.
    • Journal Title

      Periodontol 2000 52(1)

      Pages: 38-52

    • Peer Reviewed
  • [Journal Article] Proteomics of Porphyromonas gingivalis within a model oral microbial community2009

    • Author(s)
      Kuboniwa, M., E.L. Hendrickson, Q. Xia, T. Wang, H. Xie, M. Hackett, R.J. Lamont
    • Journal Title

      BMC Microbiol 19(9)

      Pages: 98

    • Peer Reviewed
  • [Journal Article] Homotypic biofilm structure of Porphyromonas gingivalis is affected by FimA type variations2009

    • Author(s)
      Kuboniwa, M., A. Amano, H. Inaba, E. Hashino, S. Shizukuishi.
    • Journal Title

      Oral Microbiol Immunol 24(3)

      Pages: 260-3

    • Peer Reviewed
  • [Journal Article] Distinct roles of long/short fimbriae and gingipains in homotypic biofilm development by Porphyromonas gingivalis2009

    • Author(s)
      Kuboniwa, M., A. Amano, E. Hashino, Y. Yamamoto, H. Inaba, N. Hamada, K. Nakayama, G.D. Tribble, R.J. Lamont, S. Shizukuishi.
    • Journal Title

      BMC Microbiol 26(9)

      Pages: 105

    • Peer Reviewed
  • [Journal Article] Porphyromonas gingivalis invades human trophoblasts and inhibits proliferation by inducing G1 arrest and apoptosis2009

    • Author(s)
      Inaba, H., M. Kuboniwa, B. Bainbridge, O. Yilmaz, J. Katz, K.T. Shiverick, A. Amano, R.J. Lamont.
    • Journal Title

      Cell Microbiol 11(10)

      Pages: 1517-32

    • Peer Reviewed
  • [Journal Article] Identification of binding domain of Streptococcus oralis glyceraldehyde-3-phosphate dehydrogenase for Porphyromonas gingivalis major fimbriae2009

    • Author(s)
      Nagata, H., M. Iwasaki, K. Maeda, M. Kuboniwa, E. Hashino, M. Toe, N. Minamino, H. Kuwahara, S. Shizukuishi.
    • Journal Title

      Infect Immun 77(11)

      Pages: 5130-8

    • Peer Reviewed
  • [Journal Article] gingivalis accelerates gingival epithelial cell progression through the cell cycle2008

    • Author(s)
      Kuboniwa, M., Y. Hasegawa, S. Mao, S. Shizukuishi, A. Amano, R.J. Lamont, O. Yilmaz. P.
    • Journal Title

      Microbes Infect 10(2)

      Pages: 122-8

    • Peer Reviewed
  • [Journal Article] Heterogenic virulence and related factors among clinical isolates of Porphyromonas gingivalis with type II fimbriae2008

    • Author(s)
      Inaba, H., K. Nakano, T. Kato, R. Nomura, S. Kawai, M. Kuboniwa, K. Ishihara, T. Ooshima, A. Amano.
    • Journal Title

      Oral Microbiol Immunol 23(1)

      Pages: 29-35

    • Peer Reviewed
  • [Journal Article] Effect of eucalyptus extract chewing gum on periodontal health: a double-masked, randomized trial2008

    • Author(s)
      Nagata, H., Y. Inagaki, M. Tanaka, M. Ojima, K. Kataoka, M. Kuboniwa, N. Nishida, K. Shimizu, K. Osawa, S. Shizukuishi.
    • Journal Title

      J Periodontol 79(8)

      Pages: 1378-85

    • Peer Reviewed
  • [Journal Article] A Porphyromonas gingivalis tyrosine phosphatase is a multifunctional regulator of virulence attributes2008

    • Author(s)
      Maeda, K., G.D. Tribble, C.M. Tucker, C. Anaya, S. Shizukuishi, J.P. Lewis, D.R. Demuth, R.J. Lamont.
    • Journal Title

      Mol Microbiol 69(5)

      Pages: 1153-64

    • Peer Reviewed
  • [Journal Article] A novel adenovirus expressing Flt3 ligand enhances mucosal immunity by inducing mature nasopharyngeal-associated lymphoreticular tissue dendritic cell migration2008

    • Author(s)
      Sekine, S., K. Kataoka, Y. Fukuyama, Y. Adachi, J. Davydova, M. Yamamoto, R. Kobayashi, K. Fujihashi, H. Suzuki, D.T. Curiel, S. Shizukuishi, J.R. McGhee, K. Fujihashi.
    • Journal Title

      J Immunol 180(12)

      Pages: 8126-34

    • Peer Reviewed
  • [Presentation] Proteomics of Porphyromonas gingivalis within a model oral microbial community2009

    • Author(s)
      Kuboniwa, M., Hendrickson, E.L., Xie, H., Hashino, E., Shizukuishi, S., Hackett, M., Lamont, R.J.
    • Organizer
      The 2009 Gordon Research Conference on Periodontal Disease
    • Place of Presentation
      Colby-Sawyer College, NH, USA
    • Year and Date
      20090802-20090807
  • [Presentation] エリスリトールによるPorphyromonas gingivalis発現タンパク質の変化に関する網羅的解析2009

    • Author(s)
      橋野恵衣, 久保庭雅恵, 山本裕美子, 末松和博, 長洋, 雫石聰
    • Organizer
      第52回春季日本歯周病学会学術大会
    • Place of Presentation
      岡山市
    • Year and Date
      20090515-20090516
  • [Presentation] Streptococcus oralis GAPDH peptide inhibits Porphyromonas gingivalis biofilm formation2009

    • Author(s)
      Nagata, H., Iwasaki, M., Kuboniwa, M., Maeda, K., Sekine, S., Shizukuishi, S.
    • Organizer
      The 87th General Session & Exhibition of the IADR
    • Place of Presentation
      Maiami, FL, USA
    • Year and Date
      20090401-20070404
  • [Presentation] デンタルバイオフィルム形成に及ぼすStreptococcus oralis GAPDHペプチドの影響2009

    • Author(s)
      岩崎未央, 橋野恵衣, 東江正裕, 永田英樹, 久保庭雅恵, 雫石聰
    • Organizer
      第20回日本口腔衛生学会近畿・中国・四国地方会総会
    • Place of Presentation
      広島市
    • Year and Date
      2009-06-21
  • [Presentation] エリスリトールは歯周病原性細菌の揮発性硫化物産生を抑制する2009

    • Author(s)
      橋野恵衣, 久保庭雅恵, 東江正裕, 関根伸一, 田中宗雄, 雫石聰
    • Organizer
      第20回日本口腔衛生学会近畿・中国・四国地方会総会
    • Place of Presentation
      広島市
    • Year and Date
      2009-06-21
  • [Presentation] Distinct roles of major/minor fimbriae and gingipains in homotypic biofilm development by Porphyromonas gingivalis. Biofilms III2008

    • Author(s)
      Kuboniwa, M., Amano, A., Hashino, E., Shizukuishi, S.
    • Organizer
      3rd International Conference
    • Place of Presentation
      Munich, Germany
    • Year and Date
      20081006-20081008
  • [Presentation] Porphyromonas gingivalisのバイオフィルム形成に及ぼすGAPDHペプチドの阻害効果2008

    • Author(s)
      岩崎未央, 永田英樹, 久保庭雅恵, 前田和彦, 橋野恵衣, 雫石聰
    • Organizer
      第57回日本口腔衛生学会・総会
    • Place of Presentation
      さいたま市
    • Year and Date
      20081002-20081004
  • [Presentation] Role of P. gingivalis PtpA tyrosine phosphatase in exopolysaccharide production2008

    • Author(s)
      Tucker, C.M., Maeda, K., Tribble, G.D., Hagerty D., Bainbridge B., Lamont R.J.
    • Organizer
      86th General session & exhibition of the IADR
    • Place of Presentation
      Toronto, Canada
    • Year and Date
      20080702-20080705
  • [Presentation] Role of Streptococcus gordonii Cbe protein in periodontopathic biofilm development2008

    • Author(s)
      Kuboniwa, M., Hashino, E., Yamamoto, Y., Lamont, R.J., Shizukuishi, S.
    • Organizer
      86th General session & exhibition of the IADR
    • Place of Presentation
      Toronto, Canada
    • Year and Date
      20080702-20070705
  • [Presentation] Porphyromonas gingivalis線毛への口腔レンサ球菌菌体表層glyceraldehyde-3-phosphate dehydrogenaseの結合部位の解析2008

    • Author(s)
      岩崎未央, 永田英樹, 前田和彦, 久保庭雅恵, 橋野恵衣, 雫石聰
    • Organizer
      大阪大学歯学会第106回例会
    • Place of Presentation
      吹田市
    • Year and Date
      2008-07-17
  • [Book] Heterotypic Streptococcus gordonii-Porphyromonas gingivalis communities: formation, gene regulation and development2010

    • Author(s)
      Kuboniwa, M., R. J. Lamont Chapter
    • Publisher
      Genomic inquiries into oral bacterial communities(in press)

URL: 

Published: 2011-06-18   Modified: 2016-04-21  

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