2010 Fiscal Year Final Research Report
Effects of cadmium in tobacco on the differentiation of periodontal tissues ; Analysis on the destruction mechanism of periodontal tissues
| Project/Area Number |
20592473
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Social dentistry
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| Research Institution | The Nippon Dental University |
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Principal Investigator |
SATO Tsutomu The Nippon Dental University, 歯科衛生学科, 教授 (60130671)
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Tomoko 日本歯科大学, 生命歯学部, 講師 (70307958)
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| Project Period (FY) |
2008 – 2010
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| Keywords | タバコ / カドミウム / 歯周組織 / 細胞分化 / シグナル伝達 / アポトーシス |
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| Research Abstract |
To clarify the mechanism of Cd to destroy periodontal tissues、 several kind of cells including the stem cells derived from dental pulp were studied. The sensitivity of these cells、i.e. stem cells from human dental pulp、mouse embryonic stem cells、human gingival keratinocytes and fibroblasts、 to Cd were different among cells by DNA synthesis and MTT assay. Cd at concentration of 10-3mM induced apoptosis in DPS cells. The rate of apoptosis cells were 40-50 %. It was also suggested that Cd triggered the mitochondorial pathway and the cell membrane receptors causing apoptosis in DPS cells.
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