2010 Fiscal Year Final Research Report
Generation, differentiation and integration of neural stem cells into neural circuits in the adult olfactory system
| Project/Area Number |
20610004
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Stem cell biology and medical science
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| Research Institution | Nara Medical University |
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Principal Investigator |
TSUBOI Akio Nara Medical University, 医学部, 教授 (20163868)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIHARA Seiichi 奈良県立医科大学, 医学部, 助教 (90360669)
TAKAHASHI Hiroo 奈良県立医科大学, 医学部, 助教 (20390685)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 嗅覚神経系 |
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| Research Abstract |
Olfactory sensory neurons are generated from neural stem cells (NSCs) throughout life. Neurosphere assays are standard studying these NSCs in normal tissues. To isolate NSCs) from the mouse olfactory epithelium, we made neurospheres from the olfactory mucosa by a serum-free culture system. Then, we succeeded to differentiate those to olfactory neurons and astrocytes in media, not supplemented with mitogens such as EGF and FGF. Further, olfactory bulb (OB) interneurons are also generated from NSCs throughout the life. Next, by using the lentiviral system, we have analyzed the sensory input-dependent development of OB interneurons. We injected the lentivirrus (CMV promoter-gapEYFP gene) into the SVZ at neonatal stage, followed by naris occlusion.We found that the odorant-induced activity is necessary for the dendrite elongation and dendrite arborization of OB interneurons.
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[Journal Article] Sequential arrival and graded secretion of Sema3F by olfactory neuron axons specify map topography at the bulb.2010
Author(s)
Takeuchi H, Inokuchi K, Aoki M, Suto F, Tsuboi A, Matsuda I, Suzuki M, Aiba A, Serizawa S, 吉原誠一, Fujisawa H, Sakano H
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Journal Title
Cell 141
Pages: 1056-1067
Peer Reviewed
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[Journal Article] Chondroitin sulfate demarcates astrocytic territories in the mammalian cerebral cortex.2010
Author(s)
Horii-Hayashi N, Tatsumi K, Matsusue Y, Okuda H, Okuda A, Hayashi M, Yano H, Tsuboi A, Nishi M, Yoshikawa M, Wanaka A.
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Journal Title
Neuroscience Letters 483
Pages: 67-72
Peer Reviewed
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