Analysis of vacuole formation by small organic molecules

2010 Fiscal Year Final Research Report

• Project/Area Number: 20611007
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Chemical biology
• Research Institution: Kyoto University
• Principal Investigator: KAWAZOE Yoshinori Kyoto University, 化学研究所, 助教 (20402927)
• Project Period (FY): 2008 – 2010
• Keywords: オルガネラ形成 / 液胞 / 小分子有機化合物

Research Abstract

I discovered a small organic molecule that induces a huge vacuoles in mammalian cells. The aim of this study is elucidating a molecular mechanism of vacuolation by means of this compound as a tool. To examine the vacuole origin, I conducted a immuno-fluorescense microscopic study and found that localizations of AAE1 and M6PR, early and late endosome marker protein, respectively, was different from those under normal state. And I found that endosomes are enlarged by fusion each other. Furthermore, the compound was appeared to induce autophagy.

Research Products

• [Journal Article] Small-molecule fluorescent probes for specific RNA targets. (2011)
  • Author(s): Murata, A., Sato, S., Kawazoe, Y., Uesugi, M.
  • Journal Title: Chem.Commun. 47 Pages: 4712-4714
  • Peer Reviewed
• [Journal Article] Deactivation of STAT6 through serine 707 phosphorilation by JNK. (2011)
  • Author(s): Shirakawa, T., Kawazoe, Y., Tsujikawa, T., Jung, D., Sato, S., Uesugi, M.
  • Journal Title: J.Biol.Chem. 286 Pages: 4003-4010
  • Peer Reviewed
• [Journal Article] A novel screen using the Reck tumor suppressor gene promoter detects both conventional and metastasis-suppressing anticancer drugs. (2010)
  • Author(s): Murai, R., Yoshida, Y., Muraguchi, T., Nishimoto, E., Morioka, Y., Kitayama, H., Kondoh, S., Kawazoe, Y., Uesugi, M., Noda, M.
  • Journal Title: Onco Target 1 Pages: 252-264
  • Peer Reviewed
• [Journal Article] A Small Molecule that Blocks Fat Synthesis by Inhibiting the Activation of SREBP. (2009)
  • Author(s): Kamisuki, S., Mao, Q., Abu-Eliheiga, L., Gu, Z., Kugimiya, A., Kwon, Y., Shinohara, T., Kawazoe, Y., Sato, S. Asakura, K., Choo, H., Sakai, J., Wakil, SJ., Uesugi, M.
  • Journal Title: Chem.Biol. 16 Pages: 882-892
  • Peer Reviewed