Basic study on a new membrane estrogen receptor and search for its selective agonist

2010 Fiscal Year Final Research Report

• Project/Area Number: 20611020
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Chemical biology
• Research Institution: University of Occupational and Environmental Health, Japan
• Principal Investigator: YANAGIHARA Nobuyuki University of Occupational and Environmental Health, Japan, 医学部, 教授 (80140896)
• Project Period (FY): 2008 – 2010
• Keywords: 細胞膜エストロゲン受容体 / 植物性エストロゲン / カテコールアミン

Research Abstract

In the present study, we examined the effects of phytoestrogens on catecholamine (CA) dynamics in cultured bovine adrenal medullary cells. Genistein, a soy phytoestrogen, and nicotine, a major component of tobacco, stimulated the function of norepinephrine transporter (NET). Nobiletin, a component of peel of citrus fruits, suppressed CA synthesis and secretion induced by acetylcholine. The present findings suggest that genistein, nicotine, and nobiletin have a pharmacological potential on cardiovascular protective effects.

Research Products

• [Journal Article] Impaired Insulin Signaling in Endothelial C ells Reduces Insulin-Induced Glucose Uptake by Skeletal Muscle (2011)
  • Author(s): Kubota T., Kubota N., Kumagai H., Yamaguchi S., Kozono H., Takahashi T., Inoue M., Itoh S., Takamoto I., Sasako T., Kumagai K., Kawai T., Hashimoto S., Kobayashi T., Sato M., Tokuyama K., Nishimura S., Tsunoda M., Ide T., Murakami K., Yamazaki T., Ezaki O., Kawamura K., Masuda H., Moroi M., Sugi K., Oike Y., Shimokawa H., Yanagihara N., Tsutsui M., Terauchi Y., Tobe K., Nagai R., Kamata K., Inoue K., Kodama T., Ueki K., Kadowaki T.
  • Journal Title: Cell Metabolism (in press)
  • Peer Reviewed
• [Journal Article] Stimulatory effects of the soy phytoestrogen genistein onoradrenaline transporter and serotonin transporter activity. (2010)
  • Author(s): Toyohira Y., Ueno S., Tsutsui M., Itoh H., Sakai N., Saito N., Takahashi K., Yanagihara N.
  • Journal Title: Mol Nutr Food Res. 54(4) Pages: 516-524
  • Peer Reviewed
• [Journal Article] Upregulation of norepinephrine transporter function by prolonged exposure to nicotine in cultured bovine adrenal medullary cells (2010)
  • Author(s): Itoh H., Toyohira Y., Ueno S., Saeki S., Zhang H., Furuno Y., Takahashi K., Tsutsui M., Hachisuka K., Yanagihara N.
  • Journal Title: Naunyn-Schmiedeberg's Arch Pharmacol. 382 Pages: 235-243
  • Peer Reviewed
• [Journal Article] Dual effects nobiletin, a citrus polymethoxy flavone, on catecholamine secretion in cultured bovine adrenal medullary cells (2010)
  • Author(s): Zhang H., Toyohira Y., Ueno S., Shinohara Y., Itoh H., Furuno Y.,Yamakuni T., Tsutsui M., Takahashi K., Yanagihara N.
  • Journal Title: J. Neurochem. 114(4) Pages: 1030-1038
  • Peer Reviewed
• [Journal Article] Dual effects of lipophilic extract of Salvia miltiorrhiza (Danshen) on catecholamine secretion in cultured bovine adrenal medullary cells. (2009)
  • Author(s): Mao H, Zhang H, Wang H, Wang Y, Zhao F, Hu L, Yanagihara N, Gao X
  • Journal Title: J Ethnopharmacol. 125(1) Pages: 59-67
  • Peer Reviewed
• [Journal Article] Insights into the pharmacological potential of estrogens and phytoestrogens on catecholamine signaling. (2008)
  • Author(s): Yanagihara N., Toyohira Y., Shinohara Y
  • Journal Title: Annals of NY Academy Sciences 1129 Pages: 96-104
  • Peer Reviewed
• [Journal Article] Simvastatin inhibits catecholamine secretion and synthesis induced by acetylcholine via blocking Na^+ and Ca^<2+> influx in bovine adrenal medullary cells. (2008)
  • Author(s): Matsuda T, Toyohira Y, Ueno S, Tsutsui M, Yanagihara N.
  • Journal Title: J Pharmacol Exp Ther 327 Pages: 130-136
  • Peer Reviewed
• [Journal Article] Crucial role of nitric oxide synthases system in endothelium-dependent hyperpolarization in mice. (2008)
  • Author(s): Takai A, Morikawa K, Tsutsui M, Murayama Y, Tekes E, Yamagishi H, Ohashi J, Yada T, Yanagihara N, Shimokawa H
  • Journal Title: J Exp Med 205 Pages: 20530-2063
  • Peer Reviewed
• [Journal Article] Spontaneous myocardial infarction in mice lacking all three nitric oxide synthase. (2008)
  • Author(s): Nakata S., Tsutsui M., Shimokawa H., Morishita T., Sabanai K., Nagasaki M., Tanimoto A., Suda O., Yatera Y., Shibata K., Tasaki H., Sasaguri Y., Nakashima Y., Otuji Y., Yanagihara N.
  • Journal Title: Circulation 117 Pages: 2211-2223
  • Peer Reviewed
• [Journal Article] 植物性エストロゲンのカテコールアミン生合成・分泌への影響 (2008)
  • Author(s): 柳原延章、豊平由美子、上野晋、筒井正人、篠原優子、劉民慧
  • Journal Title: 日本薬理学雑誌 132 Pages: 150-154
  • Peer Reviewed
• [Presentation] New insights into the pharmacological potential of plant flavonoids on catecholamine signaling. (2011)
  • Author(s): Yanagihara N., Zhang H., Toyohira Y., Ueno S., Tsutsui M., Takahashi K.
  • Organizer: 第84回日本薬理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-03-23
• [Presentation] Daual effects of nobiletin, a citrus polymethoxy flavones, on catecholamine synthesis and secretion in cultured bovine adrenal medullary cells. (2011)
  • Author(s): Zhang H., Toyohira Y., Ueno S., Tsutsui M., Itoh H., Takahashi K., Yanagihara N.
  • Organizer: 第84回日本薬理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-03-22
• [Presentation] Stimulation of Noradrenaline Transporter and Serotonin Transporter Activity by Soy Phytoestrogen Genistein. (2010)
  • Author(s): Yanagihara N., Toyohira Y., Ueno S., Tsutsui M., Takahashi K.
  • Organizer: 16^<th> World Congress on Basic and Clinical Pharmacology
  • Place of Presentation: Copenhagen
  • Year and Date: 2010-07-20
• [Presentation] Effects of nobiletin, one of citrus polymethoxy flavone, on catecholamine synthesis and secretion in cultured bovine adrenal medullary cells (2010)
  • Author(s): Yanagihara N., Zhang H., Toyohira Y., Ueno S.
  • Organizer: 40^<th> Annual Meeting Society For Neuroscience
  • Place of Presentation: San Diego
  • Year and Date: 2010-03-18
• [Presentation] Daul effects of nobiletin, a citrus polymethoxy flaon catecholamine secretion in culturebovine adrenal medullary cells (2010)
  • Author(s): Zhang H., Toyohira Y., Ueno S., Tsutsui M., Yamakuni T., Takahashi K., Yanagihara N.
  • Organizer: 第83回日本薬理学会年会
  • Place of Presentation: 大阪
  • Year and Date: 2010-03-17
• [Book] 気をつけておくべき薬剤と食品・栄養剤との相互作用(下田妙子編集)(高齢者の栄養管理ガイドブック) (2010)
  • Author(s): 柳原延章
  • Total Pages: 135
  • Publisher: 文光堂