2010 Fiscal Year Final Research Report

Chemical localizome analysis using fission yeast for understanding nuclear function

Research Project

Project/Area Number	20611022
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Chemical biology
Research Institution	The Institute of Physical and Chemical Research
Principal Investigator	YASHIRODA Yoko The Institute of Physical and Chemical Research, 吉田化学遺伝学研究室, 専任研究員 (60360658)
Project Period (FY)	2008 – 2010
Keywords	分裂酵母 / ローカリゾーム / スプライシング
Research Abstract	Spliceostatin A (SSA), a methylated derivative of a potent antitumor microbial metabolite FR901464, was found to cause pre-mRNA accumulation and translation in mammalian cells. SSA inhibits splicing and nuclear retention of pre-mRNA binding to the splicing complex. In this study, I performed chemical localizome analysis using fission yeast for identifying proteins whose subcellular localizations were changed upon treatment with SSA. I found that significant number of proteins altered their localization to the nucleolus and that those proteins were involved in ribosomal biogenesis, suggesting that SSA impinges on ribosomal processing.

(3 results)