2012 Fiscal Year Final Research Report
In vivo gene function analysis using lentiviral vector
| Project/Area Number |
20670006
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (S)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Laboratory animal science
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
IKAWA Masahito 大阪大学, 微生物病研究所, 教授 (20304066)
|
|---|
| Project Period (FY) |
2008 – 2012
|
| Keywords | レンチウイルス / ジーントラップ / バイオリソース / 生殖 / 実験動物 / 不妊 / ノックアウト |
|---|
| Research Abstract |
In the present study, we developed the lentiviral vector system for random mutagenesis in mice. Next we analyzed reproductive failures found in the mice we created. Consequently, we found that genes coding testis specific proteins, Calr3, Pdilt, Tex101,and Pmis2are required for the maturation of sperm fertilizing protein, ADAM3, and male fertility. We further developed the preeclampsia model mice by placenta specific gene manipulation system.
|
