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2010 Fiscal Year Self-evaluation Report

Simultaneous recording of conformational changes and ionic currents of single-molecular ion channels reveals the relationship between membrane potentials and motions of the channels

Research Project

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Project/Area Number 20679002
Research Category

Grant-in-Aid for Young Scientists (S)

Allocation TypeSingle-year Grants
Research Field General physiology
Research InstitutionUniversity of Fukui

Principal Investigator

SHIMIZU Hirofumi  University of Fukui, 医学部, 講師 (50324158)

Project Period (FY) 2008 – 2011
Keywords生体膜 / チャネル / 1分子計測 / 放射線
Research Abstract

生体内で重要な働きをしている膜蛋白質は細胞膜または細胞内小器官にあって常に膜内外にかかる膜電位、およびその変動の影響をうけている。この生理的な状態で膜蛋白質が機能する際にどのように動いているのか。それを1分子で調べるための測定システムを確立することを目指す。具体的には、イオンチャネルを用いた1分子電流と1分子構造変化の同時計測システムの実現を目指す。

  • Research Products

    (8 results)

All 2011 2009 2008 Other

All Journal Article (1 results) Presentation (5 results) Remarks (2 results)

  • [Journal Article] Masayuki Iwamoto and Takashi Konno : Single Molecular Gating Dynamics for the KcsA Potassium Channel.

    • Author(s)
      Shigetoshi Oiki, Hirofumi Shimizu
    • Journal Title

      Advances in Chemical Physics Volume 146

  • [Presentation] Recordings of Gating Motions of the KcsA Potassium Channels in a Single Molecule Level2011

    • Author(s)
      Hirofumi Shimizu (Invited)
    • Organizer
      "New Developments in Time-Resolved Studies with Synchrotron Radiation"Structural Dynamics Workshop of the ESRF Users' Meeting
    • Place of Presentation
      Grenoble, France
    • Year and Date
      20110207-20110209
  • [Presentation] Gating motions of KcsA potassium channel detected as a movie2009

    • Author(s)
      Hirofumi Shimizu
    • Organizer
      Symposium of Molecular Ensemble 2009
    • Place of Presentation
      Wako, Saitama, Invited speaker
    • Year and Date
      20091207-20091209
  • [Presentation] Conformational Changes of Single-Molecular KcsA Potassium Channels during Gating Recorded in a Sub-Millisecond Time Resolution.2009

    • Author(s)
      Hirofumi Shimizu, Masayuki Iwamoto, Takashi Konno, Reo Sakakura, Yoshimitsu Aoki , Shigetoshi Oiki
    • Organizer
      The 47th annual meeting of the Biophysical Society Japan
    • Place of Presentation
      Tokushima
    • Year and Date
      20091030-20091101
  • [Presentation] Global Twisting Motion of Single Molecular KcsA Potassium Channel Upon Gating.2008

    • Author(s)
      Hirofumi Shimizu, Masayuki Iwamoto, Amiko Nihei, Takashi Konno, Yuji C.Sasaki, Shigetoshi Oiki
    • Organizer
      New Approaches to Complexity of Protein Dynamics by Single Molecule Measurements : Experiments and Theories
    • Place of Presentation
      Osaka Gold Poster Award 受賞
    • Year and Date
      20081207-20081209
  • [Presentation] Visualization of conformational changes of single molecular KcsA potassium channels revealed global twisting motions upon gating.2008

    • Author(s)
      Hirofumi Shimizu, Masayuki Iwamoto, Amiko Nihei, Takashi Konno, Yuji C.Sasaki, Shigetoshi Oiki
    • Organizer
      The 46th annual meeting of the Biophysical Society Japan
    • Place of Presentation
      Fukuoka
    • Year and Date
      20081203-20081205
  • [Remarks] 2010年文部科学大臣表彰若手科学者賞受賞清水啓史

  • [Remarks] イオンチャネル開閉構造変化のX線1分子計測についての研究

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Published: 2012-02-13   Modified: 2016-04-21  

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