2009 Fiscal Year Final Research Report
Identification and characterization of zebrafish mutants defective in locomotion/behavior and application to associ ated human diseases
| Project/Area Number |
20680023
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory animal science
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| Research Institution | Nagoya University |
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Principal Investigator |
HIROMI Hirata Nagoya University, 大学院・理学研究科, 助教 (60402450)
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| Project Period (FY) |
2008 – 2009
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| Keywords | ゼブラフィッシュ |
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| Research Abstract |
I identified and analyzed eight zebrafish mutants, which have defects in locomotion and behavior. For instance, mutant mi310 harbored a point mutation in PIG-U gene, which is necessary for biogenesis of GPI-anchored proteins. In mutants, GPI-anchored proteins were not generated and surface expression of voltage-gated sodium channels was perturbed, leading to loss of action potentials. As a consequence, mutant zebrafish did not show escape behavior due to hypofunction of primary sensory neurons. The corresponding gene in human is a good candidate for a responsible gene for a human motor disorder.
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[Presentation] Zebrafish ryanodine receptor mutants show slow swimming and provide a model of Multi-minicore disease.2008
Author(s)
Hiromi Hirata, Takaki Watanabe, Jun Hatakeyama, Shawn M. Sprague, Louis Saint-Amant, Ayako Nagashima, Wilson W. Cui, Weibin Zhou, John Y. Kuwada.
Organizer
The 8th International Conference on Zebrafish Development and Genetics.
Place of Presentation
Madison, Wisconsin, USA.
Year and Date
20080625-20080629
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