Molecular genetic analysis of plant organ size control

2010 Fiscal Year Final Research Report

• Project/Area Number: 20687004
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: SUGIMOTO Keiko The Institute of Physical and Chemical Research, 細胞機能研究ユニット, ユニットリーダー (30455349)
• Co-Investigator(Renkei-kenkyūsha): 吉村 美香 , 技術補佐員 ; 毛利 真理子 , 技術補佐員 ; 池田 智香 , 技術補佐員
• Project Period (FY): 2008 – 2010
• Keywords: サイズ制御 / 細胞増殖 / 細胞伸長 / 細胞分化 / シロイヌナズナ

Research Abstract

What controls size is an important question in both basic and applied biology but the intrinsic mechanism mediating this control is not well understood. In this study, we aimed at unraveling the molecular mechanism controlling organ size in plants. Using Arabidopsis as a model system, we identified novel genes regulating cell proliferation, cell expansion and cell differentiation, and provided new molecular insights into how plants control organ size through modulating these cellular processes.

Research Products

• [Journal Article] The AP2/ERF transcription factor WIND1 controls cell dedifferentiation in Arabidopsis. (2011)
  • Author(s): Iwase A, Mitsuda N, Koyama T, Hiratsu K, Kojima M, Arai T, Inoue Y, Seki M, Sakakibara H, Sugimoto K, Ohme-Takagi M.
  • Journal Title: Curr Biol 21 Pages: 508-514
  • Peer Reviewed
• [Journal Article] Developmental control of endocycles and cell growth in plants. (2010)
  • Author(s): Breuer C, Ishida T, Sugimoto K.
  • Journal Title: Curr Opin in Plant Biol 13 Pages: 654-660
  • Peer Reviewed
• [Journal Article] Auxin modulates the transition from the mitotic cycle to the endocycle in Arabidopsis. (2010)
  • Author(s): Ishida, T., Adachi, S., Yoshimura, M., Shimizu, K., Umeda, M., Sugimoto, K.
  • Journal Title: Development 137 Pages: 63-71
  • Peer Reviewed
• [Journal Article] Arabidopsis thaliana GYRB3 does not encode a DNA gyrase subunit. (2010)
  • Author(s): Evans-Roberts K, Breuer C, Wall M, Sugimoto-Shirasu K, Maxwell A.
  • Journal Title: PLoS ONE Pages: 5e9899
  • Peer Reviewed
• [Journal Article] Functional analyses of the Arabidopsis LONELY GUY family encoding cytokinin-activvng cytokinin-activily encoding cytokinin-activnzymes reveal the imme immportance of the direct activation pathway in Arabidopsis. (2009)
  • Author(s): Kuroha T, Tokunaga H, Kojima M, Umeda N, Ishida T, Nagawa S, Fukuda H, Sugimoto K, Sakakibara H.
  • Journal Title: Plant Cell 21 Pages: 3152-3169
  • Peer Reviewed
• [Journal Article] The trihelix transcription factor AtGTL1 controls ploidy-dependent cell growth in the Arabidopsis trichome. (2009)
  • Author(s): Breuer C, Kawamura A, Ichikawa T, Tominaga-Wada R, Wada T, Kondou Y, Muto Y, Matsui M, Sugimoto K.
  • Journal Title: Plant Cell 21 Pages: 2307-2322
  • Peer Reviewed
• [Journal Article] SUMO E3 ligase HIGH PLOIDY 2 regulates endocycle onset and meristem maintenance in Arabidopsis. (2009)
  • Author(s): Ishida T, Fujiwara S, Miura K, Stacey N, Yoshimura M, Schneider K, Adachi S, Minamisawa K, Umeda M, Sugimoto K.
  • Journal Title: Plant Cell 21 Pages: 2284-2297
  • Peer Reviewed
• [Presentation] DNA損傷応答がfugu2の補償作用に果たす役割 (2011)
  • Author(s): 久永哲也、FERJANI Ali、堀口吾朗、石田喬志、杉本慶子、塚谷裕一
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 仙台市
  • Year and Date: 2011-03-20
• [Presentation] 新規SUMO E3 ligase HIGH PLOIDY 2の分裂組織における機能の解析 (2011)
  • Author(s): 石田喬志, 吉村美香, 杉本慶子
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 仙台市
  • Year and Date: 2011-03-20
• [Presentation] 転写因子WIND1は植物細胞の脱分化を制御する (2011)
  • Author(s): 岩瀬哲, 杉本慶子, 高木優
  • Organizer: 第52回日本植物生理学会年会シンポジウム「オーキシン研究の新展開」
  • Place of Presentation: 仙台市
  • Year and Date: 2011-03-20
• [Presentation] More cell or more cycle? Endocycle control in plant development. (2010)
  • Author(s): Keiko Sugimoto, Takashi Ishida, Christian Breuer, Ayako Kawamura, Mika Yoshimura.
  • Organizer: 21^<st> International Conference on Arabidopsis Research
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 20100606-20100610
• [Presentation] The trihelix transcription factor GTL1 regulates ploidy-dependent cell growth in the Arabidopsis trichome. (2010)
  • Author(s): Christian Breuer, Ayako Kawamura, Takanari Ichikawa, Rumi Tominaga-Wada, Takuji Wada, Youichi Kondou, Shu Muto, Minami Matsui, Keiko Sugimoto.
  • Organizer: ICAR-International Conference for Arabidopsis Research
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 20100606-20100610
• [Presentation] Analysis of new Arabidopsis mutants with altered organ size authors. (2010)
  • Author(s): Niinuma Kanae, Breuer Christian, Kawamura Ayako, Sugimoto Keiko.
  • Organizer: 21st International Conference on Arabidopsis Research
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 20100606-20100610
• [Presentation] SUMO E3 Ligase HIGH PLOIDY2 Regulates Endocycle Onset and Meristem Maintenance in Arabidopsis. (2010)
  • Author(s): Ishida T, Fujiwara S, Miura K, Stacey N, Yoshimura M, Schneider K, Adachi S, Minamisawa K, Umeda M, Sugimoto K.
  • Organizer: 21st International Conference on Arabidopsis Research
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 20100606-20100610
• [Presentation] The trihelix Transcription Factor AtGTL1 Regulates Ploidy-dependent Cell Growth in the Arabidopsis Trichome (2010)
  • Author(s): Breuer C., 河村彩子, 杉本慶子
  • Organizer: 21st International Conference on Arabidopsis Research
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 20100606-20100610
• [Presentation] 日本植物生理学会奨励賞受賞講演、植物の核内倍化とサイズ制御の発生遺伝学的解析 (2010)
  • Author(s): 杉本慶子
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 熊本市
  • Year and Date: 20100318-20100320
• [Presentation] The Trihelix Transcription Factor GTL1 Regulates Ploidy-Dependent Cell Growth In The Arabidopsis Trichome. (2010)
  • Author(s): 河村彩子, Breuer C, 市川尚斉, 和田るみ, 和田拓治, 近藤陽一, 武藤周, 松井南, 杉本慶子
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 熊本市
  • Year and Date: 20100318-20100320
• [Presentation] シロイナズナにおける新規器官サイズ変異体の解析 (2010)
  • Author(s): 新沼協, Breuer C, 河村彩子, 杉本慶子
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 熊本市
  • Year and Date: 20100318-20100320
• [Presentation] オーキシン依存的な細胞分裂周期と核内倍加周期の切り替えによる細胞分化制御 (2010)
  • Author(s): 石田喬志, 安達澄子, 吉村美香, 清水皓平, 梅田正明, 杉本慶子
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 熊本市
  • Year and Date: 20100318-20100320
• [Presentation] PIZZA : A new player in the brassinosteroid metabolism? (2010)
  • Author(s): Schneider K., Breuer C., 河村彩子, 市川尚斉, 近藤陽一, 松井南, 杉本慶子
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 熊本市
  • Year and Date: 20100318-20100320
• [Presentation] SUMO E3 ligase HIGH PLOIDY2 regulates endocycle onset and meristem maintenance in Arabidopsis. (2010)
  • Author(s): 石田喬志, 杉本慶子
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 熊本市
  • Year and Date: 20100318-20100320
• [Presentation] オーキシン依存的な細胞分裂周期と核内倍加周期の切り替えによる細胞分化制御 (2010)
  • Author(s): 石田喬志, 安達澄子, 吉村美香, 清水皓平, 梅田正明, 杉本慶子
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 春日井市
  • Year and Date: 2010-09-09
• [Presentation] A Wound Responsive Transcription Factor WIND1 Promotes Cell Dedifferentiation in Arabidopsis. (2010)
  • Author(s): Iwase A., Mitsuda N., Koyama K., Hiratsu K, Kojima M., Arai T., Inoue Y., Seki M., Sakakibara H., Sugimoto K., Ohme-Takagi M.
  • Organizer: 21st International Conference on Arabidopsis Research, Concurrent Session 1 : Regeneration
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2010-06-07
• [Presentation] A Wound Responsive Transcription Factor WIND1 Promotes Cell Dedifferentiation in Arabidopsis. (2010)
  • Author(s): Iwase A., Mitsuda N., Sugimoto K., Ohme-Takagi M.
  • Organizer: Keystone Symposia, Receptors and Signaling in Plant Development and Biotic Interactions.
  • Place of Presentation: Tahoe City, California, USA
  • Year and Date: 2010-03-15
• [Presentation] SUMO E3 Ligase HIGH PLOIDY2/AtMMS21 Regulates Endocycle Onset and Stem Cell Maintenance in Arabidopsis (2009)
  • Author(s): 石田喬志、藤原すみれ、Stacey Nicola、吉村美香、Schneider Katja、杉本慶子
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜市
  • Year and Date: 20091209-20091212
• [Presentation] 細胞増殖と核内倍加を制御する分子機構の解析 (2009)
  • Author(s): 石田喬志、藤原すみれ、Schneider Katja、Stacey Nicola、杉本慶子
  • Organizer: 第73回日本植物学会大会
  • Place of Presentation: 山形市
  • Year and Date: 20090917-20090920
• [Presentation] EMBO conference "Frontiers of Plant Research" (2009)
  • Author(s): Keiko Sugimoto
  • Organizer: Developmental control of cell size and ploidy in plants
  • Place of Presentation: Cadiz (Spain)
  • Year and Date: 20090506-20090509
• [Presentation] Transcriptional control of cell expansion in the Arabidopsis trichome (2009)
  • Author(s): Christian Breuer、河村彩子、市川尚斉、松井南、杉本慶子
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋市
  • Year and Date: 20090321-20090324
• [Presentation] 概日時計関連因子多重機能欠損変異体が示す器官サイズ異常の解析 (2009)
  • Author(s): 新沼協、高瀬将映、塚谷裕一、溝口剛、杉本慶子
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋市
  • Year and Date: 20090321-20090324
• [Presentation] 新規核相増加変異体を用いた核内倍加抑制機構の解析 (2008)
  • Author(s): 石田喬志、Stacey Nicola、杉本慶子
  • Organizer: 日本植物学会第72回大会
  • Place of Presentation: 高知市
  • Year and Date: 20080924-20080927
• [Presentation] Topo2008 : DNA Topoisomerases in Biology and Medicine (2008)
  • Author(s): Keiko Sugimoto
  • Organizer: Functional role of DNA topoisomerase VI in plant development
  • Place of Presentation: Norwich (UK)
  • Year and Date: 20080720-20080724
• [Presentation] Japan-Korea Symposium on Plant Growth and Signal Transduction, Genetic control of cell size and ploidy in Arabidopsis (2008)
  • Author(s): Keiko Sugimoto
  • Organizer: Japan-Korea Symposium
  • Place of Presentation: 横浜市
  • Year and Date: 20080609-20080610
• [Presentation] Functional characterization of cytokinin-activating enzymes in Arabidopsis (2008)
  • Author(s): 黒羽剛、徳永浩樹、小嶋美紀子、上田七重、石田喬志、名川信吾、福田裕穂、杉本慶子、榊原均
  • Organizer: 第10回記念植物バイオセミナー
  • Place of Presentation: 春日井市
  • Year and Date: 2008-10-08
• [Book] 科学と生物(核内倍加と細胞成長を規定する発生制御) (2011)
  • Author(s): 石田喬志, Chrisitain Breuer, 杉本慶子
  • Publisher: in press
• [Book] 生物の科学 遺伝((特集I植物染色体の最前線)核内倍加-自発的に倍加してしまう染色体の制御) (2009)
  • Author(s): 杉本慶子・石田喬志
• [Book] In The Plant Growth Regulators.(Plant cell growth signalling and its link to ploidy.)(Edited by Beemster, G., Bogre, L.) (2008)
  • Author(s): Yoshizumi T., Breuer C., Minami M., Sugimoto-Shirasu K.
  • Total Pages: 107-125
  • Publisher: Springer, USA
• [Remarks] ホームページ等 プレスリリース
• [Remarks] 植物細胞の脱分化を促進するスイッチ因子を発見(2009年3月11日)
• [Remarks] 植物細胞の大きさを調節する新たな遺伝子「GTL1」を発見(2009年9月1日)
• [Remarks] 細胞分裂の調節に必須の新しい「HPY2」遺伝子を発見(2009年8月11日)