Analysis of host factors involved in the defense network against plant viruses

2010 Fiscal Year Self-evaluation Report

• Project/Area Number: 20688002
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Plant pathology
• Research Institution: Hokkaido University
• Principal Investigator: NAKAHARA Kenji Hokkaido University, 大学院・農学研究院, 助教 (90315606)
• Project Period (FY): 2008 – 2011
• Keywords: PAMPs / RNAi / 自然免疫 / 植物ウイルス

Research Abstract

植物は自然免疫を多層的に発達させ、互いに連携したネットワークとして病原微生物に対抗していることが明らかになりつつある。ウイルスに対してもこのような防御ネットワークが発達していることが想定されているが現在の知見ではうまく説明できない。本研究でタバコのカルモジュリン様タンパクrgs-CaMを介した未知の防御機構がRNAサイレンシングや他の防御機構と連携してウイルスに対する防御ネットワークを形成していることを明らかにする。

Research Products

• [Journal Article] Sensitive PCR-based detection of Apple chlorotic leaf spot virus heterogenous in apple trees (2011)
  • Author(s): Kenji S.Nakahara, Kouji Yoshida, Tsutae Ito, Kouichi Suzaki, Nobuyuki Yoshikawa
  • Journal Title: Japan Agricultural Research Quarterly in press
  • Peer Reviewed
• [Journal Article] Involvement of the P1 Cistron in Overcoming eIF4E-Mediated Recessive Resistance Against Clover yellow vein virus in Pea (2010)
  • Author(s): Kenji S.Nakahara, Ryoko Shimada, Sun-Hee Choi, Haruko Yamamoto, Jun Shao, Ichiro Uyeda
  • Journal Title: Molecular Plant Microbe Interactions 23 Pages: 1460-1469
  • Peer Reviewed
• [Journal Article] Preall1, Priya Bellare1, 5, Erik J.Sontheimer1 & Richard W.Carthew, Silencing by small RNAs is linked to endosomal trafficking (2009)
  • Author(s): Young Sik Lee, Sigal Pressman, Arlise P.Andress, Kevin Kim, Jamie L.White, Justin J.Cassidy, Xin Li1, Kim Lubell, Do Hwan Lim, Ik Sang Cho, Kenji Nakahara, Jonathan B.
  • Journal Title: Nature Cell Biology 11 Pages: 1150-1156
  • Peer Reviewed
• [Journal Article] The cyv-2 resistance to Clover yellow vein virus in pea is controlled by the eukaryotic initiation factor 4E (2009)
  • Author(s): Marcelo Andrade, Yosuke Abe, Kenji S.Nakahara, Ichiro Uyeda
  • Journal Title: Journal of General Plant Pathology 75 Pages: 241-249
  • Peer Reviewed
• [Journal Article] Activation of the Salicylic Acid Signaling Pathway Enhances Clover yellow vein virus Virulence in Susceptible Pea Cultivars (2009)
  • Author(s): Go Atsumi, Uiko Kagaya, Hiroaki Kitazawa, Kenji S.Nakahara, Ichiro Uyeda
  • Journal Title: Molecular Plant Microbe Interactions 22 Pages: 166-175
  • Peer Reviewed
• [Presentation] Counterdefense ag ainst Viral RNAi Suppressors in Tob acco (2011)
  • Author(s): Kenji Nakahara
  • Organizer: Keystone SYMPOSIA Mechani sm and Biology of Silencing
  • Place of Presentation: Monter ey, California, USA(招待講演)
  • Year and Date: 20110320-20110325
• [Presentation] タバコのウイルスに対する基礎免疫におけるカルモジュリン様タンパクrgs-CaMの役割1 (2011)
  • Author(s): 中原健二
  • Organizer: 平成23年度日本植物病理学会大会
  • Place of Presentation: 東京農工大学(府中市)
  • Year and Date: 2011-03-29
• [Presentation] Effect of rgs-CaM overexpression on PVY accumulation in transgenic tobacco plants (2010)
  • Author(s): Syota Yamada
  • Organizer: Keystone symposia RNA silencing mechanisms in plants
  • Place of Presentation: Santa Fe, New Mexico, USA
  • Year and Date: 20100221-26
• [Presentation] eukaryotic initiation factor 4E involved in a recessive resistance to Clover yellow vein virus in pea carrying cyv2, International Congress of Virology (2008)
  • Author(s): Kenji Nakahara
  • Organizer: The International Union of Microbiological Societies
  • Place of Presentation: Istanbul, ISTANBUL CONVENTION AND EXHIBITION CENTRE
  • Year and Date: 20080810-20080815