2010 Fiscal Year Final Research Report
Symbiotic phosphate acquisition in mycorrhizal legume
| Project/Area Number |
20688004
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Plant nutrition/Soil science
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| Research Institution | Shinshu University |
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Principal Investigator |
SAITO Katsuharu Shinshu University, 農学部, 准教授 (40444244)
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| Project Period (FY) |
2008 – 2010
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| Keywords | アーバスキュラー菌根菌 / ポリリン酸 / ミヤコグサ / 酸性ホスファターゼ / 電子顕微鏡 / リン酸 / 共生 / 樹枝状体 |
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| Research Abstract |
We analyzed a phosphate translocation mechanism of arbuscular mycorrhiza by using methods of immunocytochemistry and molecular biology. Polyphosphates were detected in vacuoles and cell walls of intraradical hyphae, but were hardly detected in arbuscules. As an acid phosphatase activity was detected in peri-arbuscular spaces, the polyphosphate in cell wall of arbuscules may be hydrolyzed by the acid phosphatase. We also identified a purple acid phosphatase gene LjPAP3 in Lotus japonicus, which specifically expressed in cortical cells including arbuscules.
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