2009 Fiscal Year Final Research Report
Two-photon microscopic analysis of B cell differentiation in the antibody response
| Project/Area Number |
20689011
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Immunology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
OKADA Takaharu The Institute of Physical and Chemical Research, 免疫細胞動態研究ユニット, ユニットリーダー (50452272)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 免疫学 / 細胞・組織 / イメージング |
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| Research Abstract |
In order to track germinal center B cell differentiation in antibody responses, a reporter mouse was established to quantify BCL6 protein expression in live cells. Two-photon imaging experiments revealed that migration of differentiating B cells and helper T cells into the germinal center was impaired by attenuated function of BCL6. In addition, integrins were shown to be involved in conjugation of cognate antigen-engaged B cells and T cells in the lymph node, and the stromal cell network was suggested to be important for migration of the B-T conjugates.
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[Journal Article] Rapl controls lymphocyte adhesion cascade and interstitial migration within lymph nodes in RAPL-dependent and -independent manners2010
Author(s)
Ebisuno Y., Katagiri K., Katakai T., Ueda Y., Nemoto T., Inada H., Nabekura J., Okada T., Kannagi R., Tanaka T., Miyasaka M., Hogg N., Kinashi T
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Journal Title
Blood 115巻
Pages: 804-814
Peer Reviewed
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[Journal Article] TRPM2-mediated Ca2+ influx induces chemokine production in monocytes that aggravates inflammatory neutrophil infiltration2008
Author(s)
Yamamoto S., Shimizu S., Kiyonaka S., Takahashi N., Wajima T., Hara Y., Negoro T., Hiroi T., Kiuchi Y., Okada T., Kaneko S., Lange I., Fleig A., Penner R., Nishi M., Takeshima H., Mori Y
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Journal Title
Nat. Med 14巻
Pages: 738-747
Peer Reviewed
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