Proteomic analysis of telencephalin-binding proteins regulating synapse formation

2009 Fiscal Year Final Research Report

• Project/Area Number: 20700307
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neuroscience in general
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: FURUTANI Yutaka The Institute of Physical and Chemical Research, シナプス分子機構研究チーム, 研究員 (80392108)
• Project Period (FY): 2008 – 2009
• Keywords: テレンセファリン / プロテオミクス / 樹状突起フィロポディア / スパイン / シナプス形成 / 細胞接着分子 / 神経細胞

Research Abstract

Telencephalin is specifically expressed by spiny neurons within the telencephalon and abundantly localized to dendritic filopodia. We identified an extracellular matrix molecule, vitronectin, as a specific ligand for telencephalin. When vitronectin-coated beads were added to cultured hippocampal neurons, they adhered onto dendrites and strongly induced telencephalin accumulation. Then, we performed proteomic analysis of neuronal complex bound to vitronectin-coated beads. We determined several signaling molecules localized to dendritic filopodia.

Research Products

• [Journal Article] Identification and Characterization of nCLP2, a Novel C1q Family Protein Expressed in the Central Nervous System (2010)
  • Author(s): C. Shimono, R. Manabe, T. Yamada, S. Fukuda, J. Kawai, Y. Furutani, K. Tsutsui, K. Ikenaka, Y. Hayashizaki, K. Sekiguchi
  • Journal Title: J. Biochem. 147 Pages: 565-579
  • Peer Reviewed
• [Journal Article] Evolution of trappin genes in mammals (2010)
  • Author(s): A. Kato, AP Rooney, Y. Furutani, S. Hirose
  • Journal Title: BMC Evol Biol. 29
  • Peer Reviewed
• [Journal Article] Transcriptome-based Systematic Identification of Extracellular Matrix Proteins (2008)
  • Author(s): R. Manabe, K. Tsutsui, T. Yamada, M. Kimura, I. Nakano, C. Shimono, N. Sanzen, Y. Furutani, T. Fukuda, Y. Oguri, K. Shimamoto, D. Kiyozumi, Y. Sato, Y. Sado, H. Senoo, S. Yamashina, S. Fukuda, J. Kawai, N. Sugiura, K. Kimata, Y. Hayashizaki, K. Sekiguchi
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A. 105 Pages: 12849-12854
  • Peer Reviewed
• [Presentation] Interaction between Telencephalin and Vitronectin Induces Phagocytic Synapses on Neuronal Dendrites (2009)
  • Author(s): Y. Furutani, M. Kawasaki, H. Matsuno, K. Mori, Y. Yoshihara
  • Organizer: The 39th Annual Meeting of the Society for Neuroscience
  • Place of Presentation: Chicago
  • Year and Date: 2009-10-20
• [Presentation] Phagocytic Synapses on Neurons Induced by Telencephalin-Vitronectin Interaction (2009)
  • Author(s): Y. Furutani, M. Kawasaki, H. Matsuno, K. Mori, Y. Yoshihara
  • Organizer: The 32nd Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: Nagoya
  • Year and Date: 2009-09-16