2009 Fiscal Year Final Research Report
Relationships between Zic and folic acid metabolic pathway on spina biphida
| Project/Area Number |
20700326
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
FUJIMI Takahiro The Institute of Physical and Chemical Research, 行動発達障害研究チーム, 研究員 (80322452)
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| Project Period (FY) |
2008 – 2009
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| Keywords | Zic / 二分脊椎症 / MTHFR / shroom3 |
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| Research Abstract |
The aim of this study is to elucidate the relationship between Zic (one of the causative gene of spina bifida) and folic acid (risk reliever of spina bifida). Xenopus embryos were used for this study because of the easy observation and handling of early embryo. Spatiotemporal expression patterns of MTHFR gene (significant gene of folic acid metabolism) were overlapped with those of XZic2 gene. Knockdown (KD) analysis with moropholino oligo showed that both MTHFR and XZic2 KDs induced the reduction of shroom3 expression, which is significant for neural tube closure.
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