Redox signaling in Semaphorin-mediated axon guidance

2009 Fiscal Year Final Research Report

• Project/Area Number: 20700339
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Tokyo University of Pharmacy and Life Science
• Principal Investigator: INATOME Ryoko Tokyo University of Pharmacy and Life Science, 生命科学部, 研究員 (90408691)
• Project Period (FY): 2008 – 2009
• Keywords: 細胞内情報伝達

Research Abstract

During neural circuit formation repulsive and attractive factors such as semaphorins and netrin play a crucial role in axon guidance. However, their intracellular mechanisms are largely unknown. We have previously identified several novel proteins named CRAM, CRAG, M-septin, MITOL, which were involved in semaphoring-mediated signaling. Although exact roles of these molecules in neural development remained unclarified, these proteins were suggested to be involved in redox signaling via reactive oxygen species (ROS). Indeed, we observed the nuclear translocation and activation of CRAG in response to ROS generated by semaphorin stimulation. This fact suggested a novel concept that axon guidance signaling is redox reaction. Further studies may make clear the molecular mechanism of redox signaling in neural development. In addition, these studies may shed light on the mechanisms and develop new therapy for neuronal degenerative disorders.

Research Products

• [Journal Article] Mitochondrial ubiquitin ligase MITOL ubiquitinates mutant SOD1 and attenuates mutant SOD1-induced ROS generation. (2009)
  • Author(s): Yonashiro, R., Sugiura, A., Miyachi, M., Fukuda, T., Matsushita, N., Inatome, R., Ogata, Y., Suzuki, T., Dohmae, N., Yanagi, S
  • Journal Title: Mol. Biol. Cell 20(21) Pages: 4524-4530
• [Presentation] 新規DISC1結合蛋白質CAMDIによる大脳皮質神経細胞移動の制御機構の解析 (2009)
  • Author(s): 杉田智子、福田敏史、稲留涼子、柳茂
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10
• [Presentation] 新規DISC1結合蛋白質CAMDIによる中心体成熟と神経細胞移動の制御 (2009)
  • Author(s): 福田敏史、杉田智子、稲留涼子、柳茂
  • Organizer: 第32回日本分子生物学会年会.
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10
• [Presentation] 新規GTPaseタンパクCRAGによる公算か機構の解明 (2009)
  • Author(s): 窪田有花、長島駿、楊立偉、柳澤香菜、井上晴満、角千春、稲留涼子、福田敏史、柳茂
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] Ubiquitination and oxidation of damaged mitochondria by mitochondrial-septin (2008)
  • Author(s): Yanagi, S., Kozaki, T., Kaneko, G., Sugiura, A., Inatome, R
  • Organizer: The 7th Annual Conference of the Japanese Society of Mitochondrial Research and Medicine
  • Place of Presentation: Kagoshima, Japan
  • Year and Date: 2008-12-21
• [Presentation] CRAGによるポリグルタミン病原因タンパクの分解機構 (2008)
  • Author(s): 長島駿、窪田有花、寅嶋崇、平井宏和、稲留涼子、福田敏史、柳茂
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11