Analysis of genomic reprogramming in mammals by utilizing inactive X chromosome.

2009 Fiscal Year Final Research Report

• Project/Area Number: 20700366
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Laboratory animal science
• Research Institution: Tottori University
• Principal Investigator: NAKANISHI Tomoko Tottori University, 医学部, 助教 (10344863)
• Project Period (FY): 2008 – 2009
• Keywords: GFP / X染色体 / 不活性 / 再プログラム化 / MacroH2A / エピゲノム変化 / クロマチン構造 / 未受精卵

Research Abstract

One of the sex chromosome, X chromosome, changes its chromatin structure during genomic reprogramming in mammalian females. We produced a construct that intensely labels inactive X chromosome by expressing GFP protein fused with MacroH2A that localizes abundantly on inactive X chromosome. It was able to trace the chromatin structure of X chromosome in culture cells and mouse early embryos by utilizing the construct. Our system would enable us to examine the process of genomic reprogramming in germ-line cells and oocytes.

Research Products

• [Journal Article] Functional roles of sperm hyaluronidases, HYAL5 and SPAM1, in fertilization in mice. (2009)
  • Author(s): Kimura, M., Kim, E., Kang, W., Yamashita, M., Saigo, M., Yamazaki, T., Nakanishi, T., Kashiwabara, S.I., Baba, T.
  • Journal Title: Biol Reprod. 81 Pages: 939-947
  • Peer Reviewed
• [Journal Article] Establishment and characterization of hepatic stem-like cell lines from normal adult rat liver. (2009)
  • Author(s): Hirata, M., Amano, K., Miyashita, A., Yasunaga, M., Nakanishi T., Sato, K.
  • Journal Title: J. Biochem. 145 Pages: 51-58
  • Peer Reviewed
• [Journal Article] Birth of normal offspring from mouse eggs activated by a phospholipase Cζprotein lacking three EF-hand domains. (2008)
  • Author(s): Nakanishi, T., Ishibashi, N., Kubota, H., Inoue, K., Ogonuki, N., Ogura, A., Kashiwabara, S., Baba, T.
  • Journal Title: J. Reprod. Dev. 54 Pages: 244-249
  • Peer Reviewed
• [Presentation] MacroH2A-EGFPを利用したマウス初期胚における不活性X染色体の追跡 (2009)
  • Author(s): 相馬淳美, 中西友子, 前田康彰, 国分一男, 堀直裕, 佐藤建三
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 兵庫県
  • Year and Date: 2009-12-09
• [Presentation] マウス初期胚における脱メチル化能の解析 (2009)
  • Author(s): 中西友子, 前田康彰, 相馬淳美, 堀直裕, 佐藤建三
  • Organizer: 特定領域研究生殖サイクル若手勉強会2009
  • Place of Presentation: 静岡県
  • Year and Date: 2009-08-27
• [Presentation] 生殖系列細胞における不活性X染色体の追跡 (2009)
  • Author(s): 相馬淳美, 中西友子, 国分一男, 堀直裕, 佐藤建三
  • Organizer: 特定領域研究生殖サイクル若手勉強会2009
  • Place of Presentation: 静岡県御
  • Year and Date: 2009-08-27
• [Presentation] MacroH2A-EGFPを利用した生殖系列細胞における不活性X染色体の標識 (2008)
  • Author(s): 相馬淳美, 中西友子, 国分一男, 堀直裕, 佐藤建三
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 兵庫県
  • Year and Date: 2008-12-09
• [Presentation] MacroH2Aを利用した不活性化X染色体の標識 (2008)
  • Author(s): 相馬淳美, 中西友子, 国分一男, 堀直裕, 佐藤建三
  • Organizer: 第1回生殖系列の世代サイクルとエピゲノムネットワーク」「第5回性分化機構の解明」合同領域会議
  • Place of Presentation: 熊本県
  • Year and Date: 2008-11-24
• [Remarks]
  • URL: http://www.med.tottori-u.ac.jp/p/igaku/gakka/seimei/bunshi/bunshi_home/