2009 Fiscal Year Final Research Report
Study on sperm preservation at room temperature in the rodents
Project/Area Number |
20700367
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Single-year Grants |
Research Field |
Laboratory animal science
|
Research Institution | Kumamoto University |
Principal Investigator |
KANEKO Takehito Kumamoto University, 生命資源研究部・支援センター, 助教 (30332878)
|
Project Period (FY) |
2008 – 2009
|
Keywords | マウス / ラット / 精子 / 保存 / ICSI |
Research Abstract |
Sperm preservation at room temperature is a powerful tool for bio-resource projects. Freeze-drying is a method which can preserve and transport sperm at room temperature without using liquid nitrogen. We studied preservation of sperm at room temperature by using freeze-drying. The fertility of freeze-dried sperm was maintained after preservation for 1 year at 4 oC. Offspring was obtained from oocytes fertilized with these sperm. Moreover, this study was described that normality of sperm chromatin is an important factor to preserve sperm at room temperature.
|
Research Products
(6 results)