2011 Fiscal Year Final Research Report
Development of Analytical Procedures for Trace-level Determination of Marine DOC by Mass Spectrometry
| Project/Area Number |
20710017
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Environmental dynamic analysis
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
HONGO Yayoi 独立行政法人理化学研究所, 物質構造解析チーム, 技師 (40435681)
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| Project Period (FY) |
2008 – 2011
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| Keywords | LC-ESI-MS / 海洋溶存態有機化合物 / 葉酸 |
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| Research Abstract |
Chemical alteration of a compound should be one of the significant factors on the formation of long-lived marine dissolved organic acid. I investigated the three reaction pathways of marine folic acid that is one of the known natural compounds in seawater. To reveal the degradation rate and detect the newly appeared compounds, a seawater incubation experiment with high-performance liquid chromatography electrospray ionization mass spectrometry(LC-ESI-MS) analysis was applied.
The degradation rate of folic acid in the seawater resembled that in the control buffer, suggesting that the marine bacterial contribution was smaller than that of chemical degradation. Photo-irradiation enhanced the degradation rate in both seawater and control medium. However, after 168 h, 67% of folic acid had decomposed without light and marine bacteria, proving that thermal degradation aside from photolysis is important for folic acid degradation. The chemical instability of folic acid was the most probable factor to control the lifetime in seawater. Accurate mass measurement, MS/MS technique and NMR analysis indicated the newly appeared compound in the incubation media as p-aminobenzoylglutamic acid(PAG).
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