2010 Fiscal Year Final Research Report
Development of a Technique for Quantitative Analysis of Heavy-ion Induced DNA Double-strand Breaks
| Project/Area Number |
20710047
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Japan Atomic Energy Agency |
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Principal Investigator |
YOKOTA Yuichiro Japan Atomic Energy Agency, 量子ビーム応用研究部門, 研究員 (30391288)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 重イオンビーム / 高LET / DNA2本鎖切断 / パルスフィールドゲル電気泳動 / 生物 / 学的効果比 / 限外ろ過 |
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| Research Abstract |
Biological effects of ionizing radiation per dose are larger in high-LET heavy ions than in low-LET radiations. As a reason of this phenomenon, radiation biologists believe that heavy ions induce localized DNA damage which is difficult to repair in irradiated cells. However, the conventional experimental techniques such as pulsed-field gel electrophoresis could not quantify radiation-induced DNA fragmentation that cuts genomic DNA shorter than 10 kbp. In the present study, I have found that most of DNA fragments shorter than 10 kbp were lost during genomic DNA preparation.
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