2011 Fiscal Year Final Research Report
Biotransformation of cellulosic biomass using a microbial mix culture
| Project/Area Number |
20710061
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Environmental technology/Environmental materials
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| Research Institution | Yamaguchi University |
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Principal Investigator |
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| Project Period (FY) |
2008 – 2011
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| Keywords | セルラーゼ / キシラナーゼ / バイオマス |
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| Research Abstract |
In this study, I tried to develop a stable microflora which can produce ethanol by saccharification of cellulosic wastes. Cellulolytic strains deposited in our laboratory showed a degrading activity to several agrowastes, but they could not make a stable microflora with yeasts. Therefore, I established a new method for screening of cellulolytic microbes and tried to isolate hitherto-uncultured cellulolytic microbes in a soil of temperate zone forest. As a result, various cellulolytic strains including putative novel species were successfully isolated. Most of them possessed xylanase activity as well as cellulose activity. Some isolates could make a stable microflora with yeasts, while only trace amount of ethanol was produced by microbial saccharification and fermentation.
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