2009 Fiscal Year Final Research Report
Development of a micro cell sorter with thermal gelation polymer solution on a 3D focusing PDMS chip which fabricated using one step 3D casting technique
| Project/Area Number |
20710103
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Microdevices/Nanodevices
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| Research Institution | Kazusa DNA Research Institute |
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Principal Investigator |
SHIRASAKI Yoshitaka Kazusa DNA Research Institute, 免疫ゲノミクス研究グループ, 基礎科学特別研究員 (70469948)
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| Project Period (FY) |
2008 – 2009
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| Keywords | PDMS / 3次元シースフロー / マイクロセルソーター |
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| Research Abstract |
In this research, 3D casting method to fabricate the 3D focusing microchannel and reservoirs in one time has been developed. The 3D focusing microchannel with the multi deck structure which enables to cast in one time has also been developed.The newly developed cell sorting microchip was evaluated with mammalian cells, and the recovery ratio and purity was improved to 100% and over 99% respectively.
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