2009 Fiscal Year Final Research Report
Measurement of chemical reaction and structural change of a protein molecule by means of localized surface plasmon resonance scattering spectroscopy.
| Project/Area Number |
20750054
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Analytical chemistry
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| Research Institution | Akita University |
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Principal Investigator |
FUJIWARA Kazuhiko Akita University, 工学資源学部, 助教 (10375222)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 生体分析 / ナノバイオ / 金ナノ粒子 / 局在表面プラズモン共鳴 |
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| Research Abstract |
A protein-protein interaction measurement technique using localized surface plasmon resonance (LSPR) scattering spectroscopy was exploited. An apparatus which consists of a flowcell, pump, total-internal reflection illumination and light detection system was constructed. A gold nanoparticle deposited sensor chip was also fabricated and used for immuno-affinity measurements between IgG and anti-IgG molecule.
An observation method for structural change of protein molecules based on plasmon coupling of gold nanoparticle was also investigated in this study. For this purpose, gold nanoparticle dimer conjugated with a protein was fabricated on glass substrate surface, and resonance light-scattering intensity was monitored. It was found that structural change rate of Heat shock protein 70 was increased in the presence of ATP.
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