2009 Fiscal Year Final Research Report
Development of hydrogen-bonding lanthanide complex that recognizes nucleic acid base and Its application for the determination of DNA sequence
| Project/Area Number |
20750055
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Analytical chemistry
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| Research Institution | University of Tsukuba |
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Principal Investigator |
YOSHIMOTO Keitaro University of Tsukuba, 大学院・数理物質科学研究科, 講師 (60392172)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 核酸 / 分子認識 / ランタノイド錯体 / 遺伝子診断・解析 / 蛍光識別 / エネルギー移動発光 |
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| Research Abstract |
We newly synthesized a nucleobase-binding ligand, AMND-DOTA, in which 2-amino-5, 7-dimethyl-1, 8-naphthyridine (AMND) was conjugated with 1, 4, 7, 10-tetraazacyclododecane-1, 4, 7-triacetic acid (DOTA) via an amide linker, and found that its terbium(III) complex (AMND-DOTA-Tb) showed green emission based on an energy transfer from the naphthyridine moiety to Tb^<3+>. The nucleobase selectivity of AMND-DOTA was not changed by the conjugation of DOTA moiety to AMND, while the nucleobase-binding ability of AMND-DOTA-Tb disappeared. Finally, a mixed solution of AMND-DOTA and AMND-DOTA-Tb enabled the luminescence-based colorimetric discrimination of single-nucleotide transversions with the naked eye at a single excitation wavelength.
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