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2009 Fiscal Year Final Research Report

Construction of DNA Nanostructures for Single Molecule Expression Analyses

Research Project

  • PDF
Project/Area Number 20750126
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Chemistry related to living body
Research InstitutionThe University of Tokyo

Principal Investigator

KUZUYA Akinori  The University of Tokyo, 大学院・工学系研究科, 助教 (00456154)

Project Period (FY) 2008 – 2009
KeywordsDNA / ナノ構造体 / タンパク / ナノアレイ / ストレプトアビジン / ナノテクノロジー
Research Abstract

We have designed and prepared a stick-like DNA origami with 9 periodical nanoscale wells that can accommodate exactly one guest molecule such as proteins per well, and successfully prepared streptavidin nanoarrays of desired patterns.

  • Research Products

    (15 results)

All 2010 2009 2008 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (8 results) Remarks (1 results)

  • [Journal Article] DNA Origami: Fold, Stick, and Beyond2010

    • Author(s)
      Akinori Kuzuya, Makoto Komiyama
    • Journal Title

      Nanoscale 2

      Pages: 310-322

    • Peer Reviewed
  • [Journal Article] Precise Site-Selective Termination of DNA Replication by Caging The 3-Position of Thymidine and Its Application to Polymerase Chain Reaction2009

    • Author(s)
      Akinori Kuzuya, Fuminori Okada, Makoto Komiyama
    • Journal Title

      Bioconjugate Chem. 20

      Pages: 1924-1929

    • Peer Reviewed
  • [Journal Article] Design and Construction of a Box-Shaped 3D-DNA Origami2009

    • Author(s)
      Akinori Kuzuya, Makoto Komiyama
    • Journal Title

      Chem. Commun.

      Pages: 4182-4184

    • Peer Reviewed
  • [Journal Article] Precisely Programmed and Robust 2D Streptavidin Nanoarray Using Periodical Nanometer-Scale Wells in DNA Origami Assembly2009

    • Author(s)
      Akinori Kuzuya, Mayumi Kimura, Kentaro Numajiri, Naohiro Koshi, Toshiyuki Ohnishi, Fuminori Okada, Makoto Komiyama
    • Journal Title

      ChemBioChem 10

      Pages: 1811-1815

    • Peer Reviewed
  • [Journal Article] Efficient Site-selective RNA Activation and Scission Achieved by Geometry Control of Acridine Intercalation in RNA/DNA Heteroduplex2009

    • Author(s)
      Akinori Kuzuya, Yun Shi, Keita Tanaka, Kenzo Machida, Makoto Komiyama
    • Journal Title

      Chem. Lett. 38

      Pages: 432-433

    • Peer Reviewed
  • [Journal Article] Single-Molecule Accommodation of Streptavidin in Nanometer-Scale Wells Formed in DNA Nanostructures2008

    • Author(s)
      Akinori Kuzuya, Kentaro Numajiri, Mayumi Kimura, Makoto Komiyama
    • Journal Title

      Nucleic Acids Symp. Ser. 52

      Pages: 681-682

    • Peer Reviewed
  • [Presentation] DNA Origamiによる箱形三次元ナノ構造体の作成2010

    • Author(s)
      葛谷, 明紀
    • Organizer
      日本化学会第90春季年会
    • Place of Presentation
      近畿大学
    • Year and Date
      2010-03-29
  • [Presentation] DNA origamiを足場にした機能性ナノマテリアルの選択的固定化2009

    • Author(s)
      葛谷, 明紀
    • Organizer
      計測自動制御学会システム・情報部門学術講演会2009
    • Place of Presentation
      東京工業大学
    • Year and Date
      2009-11-26
  • [Presentation] DNAナノ構造体に組み込んだナノメートルサイズのウェルへのゲスト分子の選択的取り込み2009

    • Author(s)
      葛谷, 明紀
    • Organizer
      第58回高分子討論会
    • Place of Presentation
      熊本大学
    • Year and Date
      2009-09-18
  • [Presentation] DNA origamiによるナノメートルサイズの箱の作製2009

    • Author(s)
      葛谷, 明紀
    • Organizer
      第24回生体機能関連化学シンポジウム
    • Place of Presentation
      九州大学
    • Year and Date
      2009-09-14
  • [Presentation] ナノメートルサイズのウェルを組み込んだDNA origamiの構築とゲスト分子のサイズ選択的取り込み2009

    • Author(s)
      葛谷, 明紀
    • Organizer
      第24回生体機能関連化学シンポジウム
    • Place of Presentation
      九州大学
    • Year and Date
      2009-09-13
  • [Presentation] Streptavidin nanoarray formed on a tape-like DNA nanostructure equipped with nanometer-sized wells2009

    • Author(s)
      KUZUYA, Akinori
    • Organizer
      FNANO2010
    • Place of Presentation
      Snowbird, Utah, USA
    • Year and Date
      2009-04-22
  • [Presentation] DNAで作ったナノメートル空間内におけるストレプトアビジンの選択的DNA修飾2008

    • Author(s)
      沼尻, 健太郎
    • Organizer
      日本化学会第89春季年会
    • Place of Presentation
      日本大学理工学部
    • Year and Date
      2008-03-30
  • [Presentation] ナノメートルサイズのウェルを複数組み込んだDNA Origamiの構築とタンパクナノアレイ作成への応用2008

    • Author(s)
      葛谷, 明紀
    • Organizer
      日本化学会第89春季年会
    • Place of Presentation
      日本大学理工学部
    • Year and Date
      2008-03-30
  • [Remarks]

    • URL

      http://www.mkomi.rcast.u-tokyo.ac.jp/member/kuzuya.htm

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Published: 2011-06-18   Modified: 2016-04-21  

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