2009 Fiscal Year Final Research Report
Identification and characterization of G-quadruplex DNA binding protein
| Project/Area Number |
20750130
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemistry related to living body
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| Research Institution | Shizuoka University |
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Principal Investigator |
OYOSHI Takanori Shizuoka University, 理学部, 助教 (80406529)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 核酸化学 / 核酸結合タンパク質 / グアニン四重鎖構造 |
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| Research Abstract |
We report that Arg-Gly-Gly domain (RGG) in EWS binds G-rich single strand DNA fold in G-quadruplex DNA while it cannot bind G-rich double and single stranded DNA. Inhibition of DNA polymerase on a template containing G-quadruplex in the presence of RGG occurred in an RGG concentration-manner by forming a stabilized G-quadruplex-RGG complex. Moreover,」 specific binding of RGG to G-quadruplex DNA is dictated by hydrogen bonding of arginine in RGG.
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