2009 Fiscal Year Final Research Report
Detection of 5-methylcytosine in DNA by functionalized oligodeoxynucleotides
| Project/Area Number |
20750134
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemistry related to living body
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| Research Institution | Kyoto University |
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Principal Investigator |
TANABE Kazuhito Kyoto University, 工学研究科, 准教授 (40346086)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 生物有機化学 |
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| Research Abstract |
Although the NQ-photosensitized oxidation accompanied by selective strand cleavage at ^mC in DNA is an attractive method for identification of the methylation site in DNA, the relatively lower sensitivity of the detection method due to the less efficiency in the photooxidation of ^mC still remains as a major subject to be improved for establishing a status of general availability. In this study, we characterized reaction mechanism of the NQ-photosensitized one-electron oxidation of 5-methyl-2-deoxycytidine (d^mC) in order to obtain insight into the design of photochemical system involving the ^mC-selective strand cleavage with high sensitivity. We confirmed that deprotonation of d^mC radical cation and formation of methyl radical species are key reaction for the present photoreaction.
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[Journal Article] Monitoring of biological one-electron reduction by ^<19>F-NMR using hypoxia selective activation of a ^<19>F-labeled indolequinone derivative2009
Author(s)
Tanabe, K.; Harada, H.; Narazaki, M.; Tanaka, K.; Inafuku, K.; Komatsu, H.; Ito, T.; Yamada, H.; Chujo, Y.; Matsuda, T.; Hiraoka, M.; Nishimoto, S.
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Journal Title
J. Am. Chem. Soc. 131
Pages: 15982-15983
Peer Reviewed
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