2009 Fiscal Year Final Research Report
PET and fluorescence imaging by using novel protein modification techniques
| Project/Area Number |
20750138
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemistry related to living body
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| Research Institution | Okayama University |
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Principal Investigator |
TAKI Masumi Okayama University, 大学院・自然科学研究科, 助教 (70362952)
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| Project Period (FY) |
2008 – 2009
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| Keywords | L / F-転移酵素・タンパク質修飾 / tRNA / アミノアシルtRNA合成酵素(ARS) / 酵素工学 / 非天然アミノ酸 / PETイメージング / 蛍光修飾 |
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| Research Abstract |
We provided a novel method for N-terminal specific fluorescence labeling and PET labeling of peptide and proteins.
Moreover, combining L/F-transferase with E. coli phenylalanyl-tRNA synthetase (ARS), we achieved non-ribosomal N-terminal-specific introduction of various kinds of nonnatural amino acids to a protein. A nonnatural amino acid is once charged onto an E. coli tRNAPhe by a mutant ARS in situ, and successively transferred from the tRNA to a target protein, namely the NEXT-A reaction.
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