2009 Fiscal Year Final Research Report
Development of Artificial DNA-binding Protein Having Osmium Complex as Specific Methylcytosine Recongintion Unit
| Project/Area Number |
20750144
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemistry related to living body
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
NOMURA Akiko The Institute of Physical and Chemical Research, 岡本独立主幹研究ユニット, ユニット研究員 (40443006)
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| Project Period (FY) |
2008 – 2009
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| Keywords | DNA / メチルシトシン / 蛋白質 / オスミウム |
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| Research Abstract |
To develop a new detection method of methylated DNA, an artificial protein has been developed that contains the zinc finger motif as a DNA-binding moiety as well as an osmium complex since the latter can bind preferentially to a methylcytosine rather than the unmethylated counterpart. Based on detailed investigation of osmium complexation with a methylcytosine, the novel protein has been synthesized via a solid-phase synthesis method and its binding ability toward methylated DNA molecules was evaluated.
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