2009 Fiscal Year Final Research Report
Transportation and Fusion of Donor Cell or Oocyte-Laden MultiphaseEmulsion Droplet On a Chip
| Project/Area Number |
20760156
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Intelligent mechanics/Mechanical systems
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| Research Institution | Tohoku University |
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Principal Investigator |
YAMANISHI Yoko Tohoku University, 大学院・工学研究科, 助教 (50384029)
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| Project Period (FY) |
2008 – 2009
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| Keywords | マイクロ・ナノデバイス / フォトリソグラフィ / 精密部品加工 |
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| Research Abstract |
This work is targeting to produce a single oocyte or donor cell laden droplet on a chip by means of droplet dispensing techniques. The novelty of the present work is that we produce particle-laden droplet by using magnetic micro-actuator (MMT) to achieve the fusion of the oocyte and donor cell on a chip. The main achievement of the work is summarized as follows :
(1) Valve-functioned MMT was successfully chopped droplet and produced size-controlled droplet together with enclosing a particle whose size of 50 μm and transported them one by one. Therefore a wide range of the size of particles or cells can be enclosed in a droplet on a chip.
(2) Hybrid type MMT was successfully fabricated by using high-accuracy Ni metal by electroplating technique. Local rigidity and magnetic property of MMT was successfully controlled and obtained 6 times displacement as large as conventional setting. The revised design chip could have laminar sheath flow or pressure balance which provided on-demand droplet production on a chip successfully.
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[Remarks] 日本機械学会若手優秀フェロー賞 木原勇輝 オンチップ細胞操作システムに関する研究 -その6:磁気マイクロツールによるマイクロドロップレットの生成-,(社)日本機械学会ロボティクス・メカトロニクス部門,p.130, 2008 年6 月7 日長野ビッグハット(長野県長野市).
