2009 Fiscal Year Final Research Report
Sequence-specific separation of functional nucleic acids by liquid-liquid extraction using molecular nano-selfassembly
| Project/Area Number |
20760515
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Properties in chemical engineering process/Transfer operation/Unit operation
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| Research Institution | Kobe University |
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Principal Investigator |
MARUYAMA Tatsuo Kobe University, 大学院・工学研究科, 准教授 (30346811)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 抽出 / 核酸 / 選択性 / 生産 / 非水系 |
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| Research Abstract |
We developed DNA-surfactant as an extractant for sequence-specific DNA extraction in a reverse-micellar system. The DNA-surfactant is a short DNA oligonucleotide conjugated with a hydrophobic oleoyl group. A DNA-surfactant is selectively hybridized to a complementary target DNA to form a double helix. The helix is transferred into a water-pool of a reverse micelle, resulting in the DNA extraction to an organic phase. As a result, we achieved sequence-selective DNA extraction.
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