2009 Fiscal Year Final Research Report
Development of Gene Expression Analysis Technique from Single-Cells by Using Micro-Tools Filled with Porous Carrier
| Project/Area Number |
20760535
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
ARAKAKI Atsushi Tokyo University of Agriculture and Technology, 大学院・共生科学技術研究院, 助教 (10367154)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 単一細胞解析 / 遺伝子発現解析 / マイクロツール / mRNA 回収 |
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| Research Abstract |
In the aim of gene expression analysis from single-cells, micro-tools enabling accurate separation of target single-cells and high efficient mRNA extraction methods were developed. Gene expression and sequence analyses from single immune cells were achieved by the methods using the micro-tools. The developed cell analysis techniques have further potential as a valuable and widely applicable for studies on gene screening and expression analyses of various kinds of cells.
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