2009 Fiscal Year Final Research Report
Development of molecular target peptide screening system by using a highly effective protein immobilization
| Project/Area Number |
20760540
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Okayama University |
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Principal Investigator |
IMANAKA Hiroyuki Okayama University, 大学院・自然科学研究科, 助教 (10379711)
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| Project Period (FY) |
2008 – 2009
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| Keywords | ペプチド / 固定化 / 相互作用 / ELISA / スクリーニング / ランダムライブラリー / 創薬 / 分子標的 |
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| Research Abstract |
We have examined a novel peptide drug screening system by which the affinity peptides that bind to functional domain of FoxP3 (target protein) could be effectively obtained by applying highly functional protein immobilization method developed in our group. As the results, functional immobilization of trimmed FoxP3 was verified at first. In addition, several affinity peptides that could significantly inhibit the binding of relevant oligo DNA to functional domain of FoxP3 were successfully isolated from T7 phage random peptide library.
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