Mechanism of the organelle streaming and positioning in plant cells

2009 Fiscal Year Final Research Report

• Project/Area Number: 20770032
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kyoto University
• Principal Investigator: UEDA Haruko Kyoto University, 大学院・理学研究科, 特定研究員(産官学連携) (90402776)
• Project Period (FY): 2008 – 2009
• Keywords: オルガネラ / 原形質流動 / 小胞体 / アクチン / ミオシン

Research Abstract

Cytoplasmic streaming is defined as an extensive intracellular motility in plants, but the mechanism remains unclear. This work demonstrates that endoplasmic reticulum (ER) dynamics is driven primarily by the ER-associated myosin XI-K, a member of a plant-specific myosin class XI. Furthermore, this work shows that the myosin XI involves in the organization of the actin filament bundles. These results suggest a model whereby dynamic three-way interactions between ER, F-actin, and myosins determine the architecture and movement patterns of the ER strands, and cause cytosol hauling traditionally defined as cytoplasmic streaming.

Research Products

• [Journal Article] Myosin-dependent endoplasmic reticulum motility and F-actin organization in plant cells (2010)
  • Author(s): Ueda, H., Yokota, E., Kutsuna, N., Shimada, T., Tamura, K., Shimmen, T., Hasezawa, S., Dolja, V.V., Hara-Nishimura, I.
  • Journal Title: Proc. Natl. Acad. Sci. USA. 107 Pages: 6894-6899
  • Peer Reviewed
• [Journal Article] Vacuolar SNAREs function in the formation of the leaf vascular network by regulating auxin distribution. (2009)
  • Author(s): Shirakawa, M. *, Ueda, H. *, Shimada, T., Nishiyama, C., Hara-Nishimura, I.
  • Journal Title: Plant Cell Physiol. 50 Pages: 1319-1328
  • Peer Reviewed
• [Journal Article] GNOM-LIKE1/ERMO1 and SEC24a/ERMO2 are required for maintenance of endoplasmic reticulum morphology in Arabidopsis thaliana. (2009)
  • Author(s): Nakano, R.T., Matsushima, R., Ueda, H., Tamura, K., Shimada, T., Li, L., Hayashi, Y., Kondo, M., Nishimura, M., Hara-Nishimura, I.
  • Journal Title: Plant Cell. 21 Pages: 3672-3685
  • Peer Reviewed
• [Presentation] アクチン繊維束の細胞内配向におけるミオシンXIの役割 (2010)
  • Author(s): 上田晴子, 横田悦雄, 朽名夏麿, 田村謙太郎, 嶋田知生, 新免輝男, 馳澤盛一郎, 西村いくこ
  • Organizer: 第51回日本植物生理学会
  • Place of Presentation: 熊本大学
  • Year and Date: 2010-03-21
• [Presentation] ミオシンは光と重力に対する環境応答のブレーキとして働く (2010)
  • Author(s): 岡本圭史, 上田晴子, 田村謙太郎, 嶋田知生, 豊田正嗣, 鈴木友美, 長谷あき, ら, 田坂昌生, 森田(寺尾)美代, 西村いくこ
  • Organizer: 第51回日本植物生理学会
  • Place of Presentation: 熊本大学
  • Year and Date: 2010-03-21
• [Presentation] ミオシン変異体の葉柄は光と重力に過敏に応答して屈曲する (2009)
  • Author(s): 岡本圭史, 上田晴子, 田村謙太郎, 嶋田知生, 鈴木友美, 長谷あき, ら, 西村いくこ
  • Organizer: 第50回日本植物生理学会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-03-23
• [Presentation] 小胞体流動機構に解明～ミオシンに着目して～ (2009)
  • Author(s): 上田晴子, 横田悦雄, 嶋田知生, 新免輝男, 西村いくこ
  • Organizer: 第50回日本植物生理学会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-03-21
• [Presentation] 小胞体の形態と細胞内分布に異常を示すermo変異体の解析 (2009)
  • Author(s): 中野亮平, 松島良, 上田晴子, 林八寿子, 田村謙太郎, 嶋田知生, 西村いくこ
  • Organizer: 第50回日本植物生理学会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-03-21
• [Remarks]
  • URL: http://www.kyoto-u.ac.jp/ja/news_data/h/h1/news6/2009/100323_1.htm