2009 Fiscal Year Final Research Report
Structural analyses for yeast Asna-1 ATPase (Get3)
| Project/Area Number |
20770078
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Structural biochemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
ATSUSHI Yamagata The University of Tokyo, 放射光連携研究機構, 助教 (20463903)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 蛋白質 / 構造生物学 / ATP加水分解酵素 / 膜タンパク質 |
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| Research Abstract |
Asna1 ATPase can insert the membrane protein containing a single transmembrane domain at the C-terminus, so called as tail anchored protein, dependent on its ATP hydrolysis. In this study, we determined the crystal structures of yeast Asna1 ATPase (Get3) in both ADP bound and nucleotide free forms. In addition, structure based biochemical studies revealed the TA protein biding site in Get3, and its conformational transition upon ATP hydrolysis.
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Research Products
(8 results)
