Investigation of the DNA recognition mechanisms of sequence-specific LINE

2010 Fiscal Year Final Research Report

• Project/Area Number: 20770085
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Structural biochemistry
• Research Institution: The University of Tokushima
• Principal Investigator: MAITA Nobuo The University of Tokushima, 疾患酵素学研究センター, 准教授 (00404046)
• Project Period (FY): 2008 – 2010
• Keywords: レトロトランスポゾン / X線結晶構造解析

Research Abstract

The final goal of this project was to obtain the crystal structure of LINE-EN : DNA to reveal how LINE-EN to bind to DNA and cleave it, but it was not completed. During the optimization for crystallization, I found that LINE-EN would form a dimer in solution, and that EN alone is not enough to make the stable complex with DNA and necessary a Myb-like domain located just downstream of EN.

Research Products

• [Journal Article] (2011)
  • Author(s): Hashiguchi T, Ose T, Kubota M, Maita N, Kamishikiryo J, Maenaka K, Yanagi Y.
  • Journal Title: Nat Struct Mol Biol 18 Pages: 135-141
• [Journal Article] (2010)
  • Author(s): Maita N, Nyirenda J, Igura M, Kamishikiryo J, Kohda D.
  • Journal Title: J Biol Chem 285 Pages: 4941-4950
• [Journal Article] (2010)
  • Author(s): Fujiwara K, Maita N, Hosaka H, Ikeda OK, Nakagawa A, Taniguchi H.
  • Journal Title: J Biol Chem 285 Pages: 9971-9980
• [Journal Article] (2010)
  • Author(s): Ikeda OK, Hosaka H, Maita N, Fujiwara K, Yoshizawa AC, Nakagawa A, Taniguchi H.
  • Journal Title: J Biol Chem 285 Pages: 18684-18692
• [Journal Article] (2008)
  • Author(s): Hashiguchi T, Kajikawa M, Maita N, Takeda M, Kuroki K, Sasaki K, Kohda D, Yanagi Y,Maenaka K.
  • Journal Title: J Virol Methods 149 Pages: 171-174
• [Journal Article] (2008)
  • Author(s): Igura M, Maita N, Kamishikiryo J, YamadaM, Obita T, Maenaka K, Kohda D.
  • Journal Title: EMBO J 27 Pages: 234-243
• [Remarks] ホームページ等
  • URL: http://mms1.ier.tokushima-u.ac.jp/article/0017777.html