2010 Fiscal Year Final Research Report
Investigation of the DNA recognition mechanisms of sequence-specific LINE
Project/Area Number |
20770085
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Single-year Grants |
Research Field |
Structural biochemistry
|
Research Institution | The University of Tokushima |
Principal Investigator |
MAITA Nobuo The University of Tokushima, 疾患酵素学研究センター, 准教授 (00404046)
|
Project Period (FY) |
2008 – 2010
|
Keywords | レトロトランスポゾン / X線結晶構造解析 |
Research Abstract |
The final goal of this project was to obtain the crystal structure of LINE-EN : DNA to reveal how LINE-EN to bind to DNA and cleave it, but it was not completed. During the optimization for crystallization, I found that LINE-EN would form a dimer in solution, and that EN alone is not enough to make the stable complex with DNA and necessary a Myb-like domain located just downstream of EN.
|
-
[Journal Article]2011
Author(s)
Hashiguchi T, Ose T, Kubota M, Maita N, Kamishikiryo J, Maenaka K, Yanagi Y.
-
Journal Title
Nat Struct Mol Biol 18
Pages: 135-141
-
[Journal Article]2010
Author(s)
Maita N, Nyirenda J, Igura M, Kamishikiryo J, Kohda D.
-
Journal Title
J Biol Chem 285
Pages: 4941-4950
-
[Journal Article]2010
Author(s)
Fujiwara K, Maita N, Hosaka H, Ikeda OK, Nakagawa A, Taniguchi H.
-
Journal Title
J Biol Chem 285
Pages: 9971-9980
-
[Journal Article]2010
Author(s)
Ikeda OK, Hosaka H, Maita N, Fujiwara K, Yoshizawa AC, Nakagawa A, Taniguchi H.
-
Journal Title
J Biol Chem 285
Pages: 18684-18692
-
[Journal Article]2008
Author(s)
Hashiguchi T, Kajikawa M, Maita N, Takeda M, Kuroki K, Sasaki K, Kohda D, Yanagi Y,Maenaka K.
-
Journal Title
J Virol Methods 149
Pages: 171-174
-
[Journal Article]2008
Author(s)
Igura M, Maita N, Kamishikiryo J, YamadaM, Obita T, Maenaka K, Kohda D.
-
Journal Title
-