Functional analysis of phospholipase Cδ3 in inner hair cells

2009 Fiscal Year Final Research Report

• Project/Area Number: 20770108
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Functional biochemistry
• Research Institution: Tokyo University of Pharmacy and Life Science
• Principal Investigator: YAMAGUCHI Hideki Tokyo University of Pharmacy and Life Science, 生命科学部, 講師 (10345035)
• Project Period (FY): 2008 – 2009
• Keywords: 細胞情報伝達機構 / イノシトールリン脂質代謝 / ホスホリパーゼC / ミオシン / 内耳有毛細胞 / エンドサイトーシス / 微絨毛形成

Research Abstract

In this study, we performed functional analyses of phospholipase Cδ3, an enzyme involved in phosphoinositide metabolism, and its binding protein Myosin VI, a motor protein involved in molecular transport. As a result, we revealed that these proteins are coexpressed in the inner hair cells in mice and colocalized on the plasma membrane in cultured cells. Furthermore, we found that phospholipase Cδ3 regulates several cellular processes which depends on Myosin VI function, such as endocytosis and microvilli formation.

Research Products

• [Journal Article] Phosphatidylinositol 4, 5-bisphosphate and PIP5-kinase Iαare required for invadopodia formation in human breast cancer cells. (2010)
  • Author(s): H. Yamaguchi†, S. Yoshida, E. Muroi, M. Kawamura, Z. Kouchi, Y. Nakamura, R. Sakai, K. Fukami
  • Journal Title: Cancer Sci. 101 Pages: 1632-1638
  • Peer Reviewed
• [Journal Article] Phospholipase C-eta2 is highly expressed in the habenula and retina. (2010)
  • Author(s): K. Kanemaru, M. Nakahara, Y. Nakamura, Y. Hashiguchi, Z. Kouchi, H. Yamaguchi, N. Oshima, H. Kiyonari, K. Fukami
  • Journal Title: Gene Expr. Patterns 10 Pages: 119-126
  • Peer Reviewed
• [Journal Article] Lipid rafts and caveolin-1 are required for invadopodia formation and extracellular matrix degradation by human breast cancer cells. (2009)
  • Author(s): H. Yamaguchi†, Y. Takeo, S. Yoshida, Z. Kouchi, Y. Nakamura, K. Fukami
  • Journal Title: Cancer Res 69 Pages: 8594-8602
  • Peer Reviewed
• [Journal Article] Cortactin regulates cofilin and N-WASP activities to control the stages of invadopodium assembly and maturation. (2009)
  • Author(s): M. Oser, H. Yamaguchi, C.C. Mader, M. Arias, V. DesMarais, J. van Rheenen, A.J. Koleske, J. Condeelis
  • Journal Title: J. Cell Biol. 186 Pages: 571-587
  • Peer Reviewed
• [Journal Article] N-WASP and cortactin are involved in invadopodium-dependent chemotaxis to EGF in breast tumor cells. (2009)
  • Author(s): V. DesMarais, H. Yamaguchi, M. Oser, L. Soon, G. Mouneimne, C. Sarmiento, R. Eddy, J. Condeelis:
  • Journal Title: Cell Motil. Cytoskeleton 66 Pages: 303-316
  • Peer Reviewed
• [Journal Article] A Mena invasion isoform potentiates EGF-induced carcinoma cell invasion and metastasis. (2008)
  • Author(s): U. Philippar, E.T. Roussos, M. Oser, H. Yamaguchi, H.D. Kim, S. Giampieri, S. Giampieri, Y. Wang, S. Goswami, J.B. Wyckoff, D.A. Lauffenburger, E. Sahai, J.S. Condeelis, F.B. Gertler
  • Journal Title: Dev. Cell 15 Pages: 813-828
  • Peer Reviewed
• [Journal Article] WASP family members and Formin proteins coordinate regulation of cell protrusions in carcinoma cells. (2008)
  • Author(s): C. Sarmiento, W. Wang, A. Dovas, H. Yamaguchi, M. Sidani, M. El-Sibai, V. DesMarais, H.A. Holman, S. Kitchen, J.M. Backer, A. Alberts, J. Condeelis
  • Journal Title: J. Cell Biol. 180 Pages: 1219-1232
  • Peer Reviewed
• [Journal Article] Membrane targeting of WAVE2 is not sufficient for WAVE2 dependent actin polymerization: a role for IRSp53 in mediating the interaction between Rac and WAVE2 (2008)
  • Author(s): W. Abou-Kheir, B. Issac, H. Yamaguchi, D. Cox
  • Journal Title: J. Cell Sci. 121 Pages: 379-390
  • Peer Reviewed
• [Journal Article] Phospholipase C-δ1 is an essential molecule downstream of Foxn1, the gene responsible for the nude mutation, in normal hair development. (2008)
  • Author(s): Y. Nakamura, M. Ichinohe, M. Hirata, H. Matsuura, T. Fujiwara, T. Igarashi, M. Nakahara, H. Yamaguchi, S. Yasugi, T. Takenawa, K. Fukami
  • Journal Title: FASEB J. 22 Pages: 841-849
  • Peer Reviewed
• [Presentation] MyosinVIの膜輸送機能におけるホスフォリパーゼCδ3の関与 (2008)
  • Author(s): 櫻井和之、平田真之、石井里佳、中村由和、河内全、山口英樹、深見希代
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] Phospholipase Cδ3はmyosin VIと結合し内耳有毛細胞において共発現する (2008)
  • Author(s): 櫻井和之、平田真之、中村由和、河内全、山口英樹、深見希代子
  • Organizer: 第60回日本細胞生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2008-06-29
• [Remarks]
  • URL: http://www.ls.toyaku.ac.jp/~genome/Site/Top.html