2009 Fiscal Year Final Research Report
A role of mitochondrial ubiquitin ligase MITOL in mitochondrial quality control.
| Project/Area Number |
20770109
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional biochemistry
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| Research Institution | Tokyo University of Pharmacy and Life Science |
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Principal Investigator |
YONASHIRO Ryo Tokyo University of Pharmacy and Life Science, 生命科学部, 助教 (60453809)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 細胞内タンパク質分解 |
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| Research Abstract |
We have previously identified a novel mitochondrial ubiquitin ligase, MITOL, which is localized in the mitochondrial outer membrane and is involved in the control of mitochondrial dynamics. In this study, we examined whether MITOL eliminates misfolded proteins localized to mitochondria. Mutant superoxide dismutase1 (mSOD1), one of misfolded proteins, has been shown to localize in mitochondria and induce mitochondrial dysfunction, possibly involving in the onset and progression of amyotrophic lateral sclerosis. We found that MITOL interacted with and ubiquitinated mSOD1. Moreover, MITOL overexpression promoted mSOD1 degradation and suppressed mSOD1-induced reactive oxygen species generation. Thus, our findings indicate that MITOL plays a protective role against mitochondrial dysfunction caused by the mitochondrial accumulation of mSOD1 via the ubiquitin-proteasome pathway.
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[Journal Article] Mitochondrial ubiquitin ligase MITOL ubiquitinates mutant SOD1 and attenuates mutant SOD1-induced ROS generation.2009
Author(s)
Yonashiro, R., Sugiura, A., Miyachi, M., Fukuda, T., Matsushita, N., Inatome, R., Ogata, Y., Suzuki, T., Dohmae, N., Yanagi, S.
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Journal Title
Mol. Biol. Cell 20(21)
Pages: 4524-4530
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