2009 Fiscal Year Final Research Report
Structural Insight for Ca channel Regulation by Calmodulin
| Project/Area Number |
20770110
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional biochemistry
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| Research Institution | Fukuoka University |
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Principal Investigator |
MORI Masayuki Fukuoka University, 医学部, 講師 (80342640)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 生理学 / シグナル伝達 / カルシウム / イメージング / 構造 |
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| Research Abstract |
The overall aim of the proposed studies was to understand the mechanism of activation, inhibition and regulations of Ca channels by Calcium-binding protein Calmodulin (CaM). The primary focus was structural determination of the calcium free form apoCaM and Channel IQ domain complex. Until today, we have a couple of crystals, those will be needed to check reproducibility and their structure. For the imaging of Ca^<2+> and CaM- channel interaction, we have succeeded to develop new system combined with Fura-2 and FRET. By using this system we could establish Ca^<2+> titrative experiment in vivo with high fidelity. We hope that this approach will be often to use in the field wide range of the physiological science to reveal Ca^<2+> related molecular dynamics.
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[Journal Article] Synergistic activation of vascular TRPC6 channel by receptor and mechanical stimulation via phospholipaseC/diacylglycerol and phospholipase A2/omega-hydroxylase/20-HETE pathways2009
Author(s)
Inoue R, Jensen LJ, Jian Z, Shi J, Hai L, Lurie AI, Henriksen FH, Salomonsson M, Morita H. Kawarabayashi Y, Mori M, Mori Y, Ito Y.
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Journal Title
Circ Res. 104
Pages: 1399-409
Peer Reviewed
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