2010 Fiscal Year Final Research Report
A genetic biochemical search and analysis for factors involved in glycosphingolipid metabolism and traffic
| Project/Area Number |
20770114
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional biochemistry
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
YAMAJI Toshiyuki National Institute of Infectious Diseases, 細胞化学部, 主任研究官 (50332309)
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| Project Period (FY) |
2008 – 2010
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| Keywords | スフィンゴ糖脂質 / 志賀毒素 / 糖転移酵素 / トランスゴルジネットワーク |
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| Research Abstract |
We performed genetic biochemical screenings with shiga toxin to look for new factors that affect glycosphingolipid (GSL) metabolism and its intracellular traffic. As a molecule to reduce Gb3, the shiga toxin receptor, we isolated the COOH-terminus region of GRINA, a member of TMBIM family, which was named GRINA-C. Overexpression of GRINA-C and FAIM2, another TMBIM family member, affected the amount of Gb3 synthase and its intracellular localization, suggesting a new type of regulation of glycosyltransferases in GSL metabolism.
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Research Products
(11 results)
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[Journal Article] Siglec-7 mediates non-apoptotic cell death independently of its immunoreceptor tyrosine-based inhibitory motifs in monocytic cell line U937.2010
Author(s)
Mitsuki, M., Nara, K., Yamaji, T., Enomoto A., Kanno, M., Yamaguchi, Y., Yamada, A., Waguri, S., Hashimoto, Y. (equal contribution)
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Journal Title
Glycobiology 20
Pages: 395-402
Peer Reviewed
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[Presentation] Binding specificity of siglec-7 prepared from various animal cell lines2009
Author(s)
Ito, S., Ito, N., Tsuchida, A., Tokuda, N., Yagi, H., Kato, K., Mitsuki, M., Yamaji, T., Hashimoto, Y., Crocker, P.R., Furukawa K.
Organizer
第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
Place of Presentation
神戸
Year and Date
2009-12-10
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